Anat Cell Biol.  2018 Jun;51(2):119-127. 10.5115/acb.2018.51.2.119.

Ameliorative effects of Moringa on cuprizone-induced memory decline in rat model of multiple sclerosis

• Omotoso GO
• Gbadamosi
• Afolabi TT
• Abdulwahab AB
• Akinlolu A

• KMID: 2421176
• DOI: http://doi.org/10.5115/acb.2018.51.2.119

Abstract

Cuprizone is a neurotoxin with copper-chelating ability used in animal model of multiple sclerosis in which oxidative stress has been documented as one of the cascade in the pathogenesis. Moringa oleifera is a phytomedicinal plant with antioxidant and neuroprotective properties. This study aimed at evaluating the ameliorative capability of M. oleifera in cuprizone-induced behavioral and histopathological alterations in the prefrontal cortex and hippocampus of Wistar rats. Four groups of rats were treated with normal saline, cuprizone, M. oleifera and a combination of M. oleifera and cuprizone, for five weeks. The rats were subjected to Morris water maze and Y-maze to assess long and short-term memory respectively. The animals were sacrificed, and brain tissues were removed for histochemical and enzyme lysate immunosorbent assay for catalase, superoxide dismutase, and nitric oxide. Cuprizone significantly induced oxidative and nitrosative stress coupled with memory decline and cortico-hippocampal neuronal deficits; however, administration of M. oleifera significantly reversed the neuropathological deficits induced by cuprizone.

Keyword

Cuprizone; Moringa oleifera; Oxidative stress; Memory

MeSH Terms

Animals
Brain
Catalase
Cuprizone
Hippocampus
Memory*
Memory, Short-Term
Models, Animal*
Moringa oleifera
Moringa*
Multiple Sclerosis*
Neurons
Nitric Oxide
Oxidative Stress
Plants
Prefrontal Cortex
Rats*
Rats, Wistar
Superoxide Dismutase
Water
Catalase
Cuprizone
Nitric Oxide
Superoxide Dismutase
Water