J Nutr Health.  2018 Aug;51(4):323-329. 10.4163/jnh.2018.51.4.323.

Anti-inflammatory activities of Scolopendra subspinipes mutilans in RAW 264.7 cells

• Park JH
• Lee SR

• KMID: 2419503
• DOI: http://doi.org/10.4163/jnh.2018.51.4.323

Abstract

PURPOSE
The dried body of Scolopendra subspinipes mutilans has long been used as a traditional Korean medicinal food, but little is known about its mechanisms of action. In this study, we investigated the anti-inflammatory activities of Scolopendra subspinipes mutilans and possible mechanisms in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells.
METHODS
Cytotoxicity of Scolopendra subspinipes mutilans extract (SSME) was measured by MTT assay, anti-inflammatory activities were analyzed by nitric oxide (NO) production, the expression of inducible NO synthase (iNOS) and the mRNA level of pro-inflammatory cytokines such as interleukin-1β (IL-1β) and interleukin-6 (IL-6). Nuclear translocation of nuclear factor-kappa B (NF-κB) p65 subunit and degradation of inhibitory kappa B (IκB) were examined by western blot.
RESULTS
SSME inhibited LPS-induced NO production and iNOS expression without cytotoxicity. Up-regulation of LPS-induced pro-inflammatory cytokines, IL-1β and IL-6 was dose dependently attenuated by SSME. Exposure of pyrrolidine dithiocarbamate, an NF-κB specific inhibitor, accelerated the inhibitory effects of SSME on NO production and iNOS expression in LPS-stimulated cells. Moreover, translocation of NF-κB from the cytosol to the nucleus and degradation of IκB were decreased by treatment with SSME in LPS-induced cells.
CONCLUSION
These results suggest that the SSME might have the inhibitory effects on inflammation, partly through inhibition of the NF-κB signaling pathway.

Keyword

inflammation; nitric oxide; NF-κB; cytokine; Scolopendra subspinipes mutilans

MeSH Terms

Blotting, Western
Cytokines
Cytosol
Inflammation
Interleukin-6
Nitric Oxide
Nitric Oxide Synthase
RAW 264.7 Cells*
RNA, Messenger
Up-Regulation
Cytokines
Interleukin-6
Nitric Oxide
Nitric Oxide Synthase
RNA, Messenger

Figure

• 

  Fig. 1 Cytotoxicity of Scolopendra subspinipes mutilans extract (SSME) in RAW 264.7 cells. The cells were treated with indicated concentration of SSE for 24 hrs. The data were presented as the mean ± SD (n=3, **p < 0.01 vs control).

• 

  Fig. 2 Effect of Scolopendra subspinipes mutilans extract (SSME) on production of NO (A), expression of iNOS protein (B) and RNA level of pro-inflammatory cytokines (C) in LPS-stimulated RAW 264.7 cells. Cells were pre-treated with indicated concentration of SSME for 1 hr, and then stimulated with LPS for 24 hrs (A & B) or for 12 hrs (C). The data were presented as the mean ± SD (n = 3, **p < 0.01 vs LPS-treated group).

• 

  Fig. 3 Inhibitory effect of Scolopendra subspinipes mutilans extract (SSME) on the NF-κB translocation to nucleus and IκB degradation (A) and NO production (B) in LPS-stimulated RAW 264.7 cells. Cells were pre-treated with indicated concentration of SSME and/or PDTC for 1 hr, and then stimulated with LPS for 75 min (A) or for 24 hrs (B). Different letters indicate significant differences among group at p < 0.01 as determined by Duncan's multiple range test.

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