Korean J Vet Res.  2018 Jun;58(2):65-72. 10.14405/kjvr.2018.58.2.65.

The effects of green tea (Camellia sinensis) flower extracton melanin synthesis in B16-F10 melanoma cells

• Dissanayake CY
• Moon HH
• Yang KM
• Lee Y
• Han CH

• KMID: 2414714
• DOI: http://doi.org/10.14405/kjvr.2018.58.2.65

Abstract

The present study observed the effects of a green tea (Camellia sinensis) flower extract (GTFE) on melanin synthesis in B16-F10 melanoma cells. GTFE exhibited antioxidant activity on 2,2-diphenyl-1-picrylhydrazyl and inhibited mushroom tyrosinase activity in a dose-dependent manner. Furthermore, GTFE significantly diminished α-melanocyte stimulating hormone (α-MSH) stimulated cellular melanin content and tyrosinase activity throughout the concentration range evaluated. Based on RNA sequencing analysis, differential gene expression patterns observed in α-MSH stimulated B16-F10 melanoma cells were normalized by the addition of GTFE. In particular, the expression levels of melanoregulin and tyrosinase genes which are key regulating genes in melanin synthesis were up-regulated by 3.5 and 3 fold respectively by α-MSH, and were normalized to control levels by the addition of GTFE. The results suggest that GTFE inhibits melanin synthesis in α-MSH stimulated B16-F10 melanoma cells by normalizing expression of genes that are essential for melanin synthesis. Overall, the results suggest that GTFE could be applied in the development of a whitening agent for the treatment of dermal hyperpigmentation.

Keyword

B16-F10 melanoma cells; RNA sequencing analysis; antioxidant effect; green tea flower; melanin synthesis inhibition; tyrosinase activity inhibition

MeSH Terms

Agaricales
Antioxidants
Flowers*
Gene Expression
Hyperpigmentation
Melanins*
Melanoma*
Monophenol Monooxygenase
Sequence Analysis, RNA
Tea*
Antioxidants
Melanins
Monophenol Monooxygenase
Tea