Gut Liver.
2017 May;11(3):392-400. 10.5009/gnl16265.
Expression of MicroRNA in Host Cells Infected with Helicobacter pylori
- Affiliations
-
- 1Department of Internal Medicine, Gachon University Gil Medical Center, Incheon, Seoul, Korea.
- 2Department of Internal Medicine, Catholic Kwandong University International St. Mary's Hospital, Incheon, Seoul, Korea.
- 3Department of Convergence Medicine and Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea. jyjeong@amc.seoul.kr
- 4Department of Gastroenterology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
- KMID: 2412213
- DOI: http://doi.org/10.5009/gnl16265
Abstract
- BACKGROUND/AIMS
MicroRNAs (miRNAs) regulate gene expression. We assess miRNA regulation by Helicobacter pylori infection and elucidate their role in H. pylori-infected gastric epithelial cells.
METHODS
The relationship between miRNA expression and DNA methylation was examined. Cells were treated with the nuclear factor-kappaB (NF-κB) inhibitor Bay 11-7082 to determine the relationship between miRNA expression and NF-κB signal transduction.
RESULTS
In the negative control cells infected with H. pylori 26695, the expression of six miRNAs was increased, whereas the expression of five miRNAs was decreased. The expression of upregulated miRNAs was increased when the host cells were treated with H. pylori and an NF-κB inhibitor. miR-127-5p, -155, and -181 were associated with increased interleukin 6 (IL-6) secretion in H. pylori infected cells treated with anti-miRNA. The expression of miR-155, -127-5p, -195, -216, -206, and -488 increased by approximately 3-fold following treatment with the methylation inhibitor Aza.
CONCLUSIONS
We found novel miR-NAs in H. pylori-infected negative control cells using miRNA microarrays. Upregulated miRNA expression was inversely related to the transcription of NF-κB. miR-195 and miR-488 appear to play a pivotal role in controlling IL-6 activity in H. pylori infection. miRNA expression in H. pylori infection was affected by methylation.
MeSH Terms
-
Adolescent
Child
Child, Preschool
DNA Methylation/drug effects
Epithelial Cells/microbiology
Female
Gastric Mucosa/microbiology
Helicobacter Infections/drug therapy/*genetics
Helicobacter pylori/*genetics
Humans
Interleukin-6/secretion
Male
MicroRNAs/*metabolism
NF-kappa B/metabolism
Nitriles/*pharmacology
Signal Transduction/genetics
Sulfones/*pharmacology
Up-Regulation/physiology
Young Adult
Interleukin-6
MicroRNAs
NF-kappa B
Nitriles
Sulfones
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