Gut Liver.  2017 Jan;11(1):156-163. 10.5009/gnl15281.

Protection Provided by a Gabexate Mesylate Thermo-Sensitive In Situ Gel for Rats with Grade III Pancreatic Trauma

• Gao H
• Song Q
• Lv F
• Wang S
• Wang Y
• Li X
• Luo Y
• Mei X
• Tang J

• KMID: 2412168
• DOI: http://doi.org/10.5009/gnl15281

Abstract

BACKGROUND/AIMS
This study investigated the protection provided by gabexate mesylate thermo-sensitive in-situ gel (GMTI) against grade III pancreatic trauma in rats.
METHODS
A grade III pancreatic trauma model with main pancreatic duct dividing was established, and the pancreas anatomical diagram, ascites, and serum biochemical indices, including amylase, lipase, C-reactive protein (CRP), interleukin 6 (IL-6), and tumor necrosis factor-α (TNF-α), were examined. The pancreas was sliced and stained with hematoxylin eosin and subjected to terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining.
RESULTS
Ascites, serum amylase, lipase, CRP, IL-6, and TNF-α levels were significantly increased in the pancreas trauma (PT) groups with prolonged trauma time and were significantly decreased after GMTI treatment. The morphological structure of the pancreas was loose, the acinus was significantly damaged, the nuclei were irregular and hyperchromatic, and there was inflammatory cell invasion in the PT group compared to the control. After GMTI treatment, the morphological structure of the pancreas was restored, and the damaged acinus and inflammatory cell invasion were decreased compared to the PT group. Moreover, the cell apoptosis index was significantly increased in the PT group and restored to the same levels as the control group after GMTI treatment.
CONCLUSIONS
GMTI, a novel formulation and drug delivery method, exhibited specific effective protection against PT with acute pancreatitis therapy and has potential value as a minimally invasive adjuvant therapy for PT with acute pancreatitis.

Keyword

Grade III pancreatic trauma; Main pancreatic duct; Thermo-sensitivity; Apoptosis; Therapeutics

MeSH Terms

Abdominal Injuries/complications
Amylases/*drug effects/metabolism
Animals
Apoptosis/*drug effects
Ascites/etiology
C-Reactive Protein/*drug effects/metabolism
Edema/etiology/metabolism/pathology
Gabexate/*pharmacology
Gels/pharmacology
In Situ Nick-End Labeling
Interleukin-6/metabolism
Lipase/*drug effects/metabolism
Male
Pancreas/*drug effects/injuries/metabolism/pathology
Rats
Rats, Sprague-Dawley
Tumor Necrosis Factor-alpha/*drug effects/metabolism
Gels
Interleukin-6
Tumor Necrosis Factor-alpha
Gabexate
C-Reactive Protein
Lipase
Amylases