Effect of Gabexate Mesilate on Exocrine Pancreatic Secreation in Rats

Kim, Sei Jong; Yoon, Chong Mann; Choi, Sung Kyu; Kim, Hyun Soo; Cho, Cheon Yeong; Rew, Jong Sun

Abstract

BACKGROUND AND AIMS: Gabexate mesilate, a serine protease inhibitor, is a synthetic compound which is not antigenic and diffuses easily. We have investigated the effect of gabexate mesilate on exocrine pancreatic secretion in rat at basal and cholecytokinin-stimulated state.
METHODS
After anesthetized male Sprague-Dwaley rats were incised in the midline of abdomen, PE-10 tube was inserted into the pancreatic duct. The secretion was collected after the ligation of the bile duct between pancreas and liver. The volume, of pancreatic secretion and the amount of bicarbonate and protein in pancreatic secretion were measured in basal and CCK-8 (3.9 pmol/hr) stimulated state with and without the administration of gabexate mesilate (30 mg/kg). Resalts: The volume was 20.1+/-1.1 u L/30 min in basal state and 58.9+/-3.9 u L/30 min in CCK-8 stimulated state. The amount of bicarbonate in basal and CCK-8 stimulated state was 30.1+/-1.3 umol/ 30 min and 193.1+/-4.3 umol/30 min, respectively. After infusion of gabexate, the volume and the amount of bicarbonate were not changed significantly. The amount of protein was 39.8+/- 1.2 u g/30 min in basa1 state and 396.0+/-6.2 u g/30 min in CCK-8 stimulated state. After the infusion of gabexate mesilate, the amount of the protein in basal and CCK-8 stimulated state was significantly decreased to 30.1+/-l.3 ug/30 min and 295.9+/-5.9 ug/30 min, respectively (p<0.05).
CONCLUSIONS
With the infusion of gabexate mesilate in both basal and CCK stimulated state of rat, only the protein in pancreatic exocrine secretions was significantly decreased.