Gut Liver.  2016 Sep;10(5):749-756. 10.5009/gnl15584.

PMK-S005 Alleviates Age-Related Gastric Acid Secretion, Inflammation, and Oxidative Status in the Rat Stomach

• Choi YJ
• Kim N
• Lee JY
• Nam RH
• Suh JH
• Lee SM
• Ham MH
• Jo HJ
• Shim YK
• Park YH
• Lee JC
• Choi YJ
• Lee HS
• Lee DH

• KMID: 2412072
• DOI: http://doi.org/10.5009/gnl15584

Abstract

BACKGROUND/AIMS
The aim of this study was to evaluate the effect of the synthetic S-allyl-l-cysteine (SAC) PMK-S005 on gastric acid secretion, inflammation, and antioxidant enzymes in aging rats.
METHODS
The rats were divided into four groups at 31 weeks of age and were continuously fed a diet containing a vehicle control, PMK-S005 (5 or 10 mg/kg), or lansoprazole (5 mg/kg). Gastric acid secretion and connective tissue thickness of the lamina propria were evaluated at 74 weeks and 2 years of age. Tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and COX-2 levels were measured by using enzyme-linked immunosorbent assays (ELISAs) or Western blot assays. Levels of antioxidant enzymes, including heme oxyganase 1 (HO-1) and NAD(P)H:quinone oxidoreductase 1 (NQO-1), were also measured.
RESULTS
As the rats aged, gastric acid secretion significantly decreased, and the connective tissue of the lamina propria increased. However, 74-week-old rats in the PMK-S005 group exhibited greater levels of gastric acid secretion than those of the control and lansoprazole groups. The increase of TNF-α, IL-1β, and COX-2 expression in 74-week and 2-year-old control rats were inhibited by PMK-S005. In addition, the decrease in HO-1 and NQO-1 protein expression that occurred with aging was inhibited by PMK-S005 in the 74-week-old rats.
CONCLUSIONS
These results suggest that PMK-S005 has therapeutic potential as an antiaging agent to ameliorate age-related gastric acid secretion, inflammation, and oxidative stress in the stomach.

Keyword

s-allyl-l-cysteine; Aging; Gastric acid; Anti-inflammation; Antioxidants

MeSH Terms

Aging/*drug effects
Animals
Antioxidants/analysis
Cyclooxygenase 2/analysis
Enzyme-Linked Immunosorbent Assay
Gastric Acid/secretion
Gastric Mucosa/secretion
Gastritis/*drug therapy/enzymology
Interleukin-1beta/analysis
Male
Oxidative Stress/*drug effects
Plant Extracts/*pharmacology
Rats
Rats, Inbred F344
Stomach/*drug effects/enzymology/metabolism
Tumor Necrosis Factor-alpha/analysis
Antioxidants
Interleukin-1beta
Plant Extracts
Tumor Necrosis Factor-alpha
Cyclooxygenase 2