Gut Liver.  2016 May;10(3):382-390. 10.5009/gnl14319.

Tryptase and Protease-Activated Receptor 2 Expression Levels in Irritable Bowel Syndrome

• Liang WJ
• Zhang G
• Luo HS
• Liang LX
• Huang D
• Zhang FC

• KMID: 2411965
• DOI: http://doi.org/10.5009/gnl14319

Abstract

BACKGROUND/AIMS
Previous studies have revealed that mast cells (MCs) may activate the protease-activated receptors and release of neuropeptides involved in the pathogenesis of irritable bowel syndrome (IBS). The levels of protease-activated receptor 2 (PAR-2) and tryptase can contribute to understanding the pathogenesis of IBS.
METHODS
Colonoscopic biopsies were performed of 38 subjects (20 with IBS-diarrhea [IBS-D], eight with IBS-constipation [IBS-C], and 10 healthy volunteers). The mRNA and protein levels of tryptase and PAR-2 were assessed by real-time PCR and Western blot. The levels of vasoactive intestinal peptide (VIP), substance P (SP), and calcitonin gene-related peptide (CGRP) were measured by immunohistochemistry, and MCs were counted by toluidine blue staining.
RESULTS
Significant increases in the mRNA expression of tryptase (p<0.05, IBS-D, IBS-C vs control) and PAR-2 (p<0.05, IBS-D, IBS-C vs control) and in the tryptase protein level (p<0.05, IBS-D, IBS-C vs control) were detected in IBS. Elevations of MCs, CGRP, VIP and SP (p<0.05, IBS-D vs control) were observed for IBS-D only.
CONCLUSIONS
Tryptase levels may upregulate the function of PAR-2, resulting in the release of neuropeptide and they were correlated with clinical symptoms associated with IBS.

Keyword

Irritable bowel syndrome; Inflammation; PAR-2; Tryptases

MeSH Terms

Abdominal Pain/etiology
Adult
Calcitonin Gene-Related Peptide/metabolism
Case-Control Studies
Colon/metabolism
Female
Humans
Irritable Bowel Syndrome/*metabolism
Male
Mast Cells/*metabolism
Middle Aged
RNA, Messenger/metabolism
Receptor, PAR-2/*metabolism
Substance P/metabolism
Tryptases/*metabolism
Vasoactive Intestinal Peptide/metabolism
RNA, Messenger
Receptor, PAR-2
Vasoactive Intestinal Peptide
Calcitonin Gene-Related Peptide
Tryptases