J Periodontal Implant Sci.  2018 Apr;48(2):70-83. 10.5051/jpis.2018.48.2.70.

Magnoliae Cortex and maize modulate Porphyromonas gingivalis-induced inflammatory reactions

Affiliations
  • 1Department of Periodontology, Dental Research Institute, Seoul National University School of Dentistry, Seoul, Korea. icrhyu@snu.ac.kr

Abstract

PURPOSE
The aim of this study was to evaluate the capacity of single and combined applications of the bark of the stems and roots of Magnolia officinalis Rehd. et Wils. (Magnoliae Cortex) and Zea mays L. (maize) to modulate inflammation in RAW 264.7 cells stimulated with Porphyromonas gingivalis.
METHODS
RAW 264.7 cells were stimulated with P. gingivalis, and Magnoliae Cortex and/or maize was added. Cytotoxicity and the capacity to modulate inflammation were determined with a methylthiazol tetrazolium (MTT) assay, nitrite production, enzyme-linked immunosorbent assay (ELISA), and western blotting.
RESULTS
Treatment with Magnoliae Cortex and/or maize inhibited nuclear transcription factor κB (NF-κB) pathway activation and nuclear p44/42 mitogen-activated protein kinase (MAPK) and inducible nitric oxide synthase (iNOS) protein expression in P. gingivalis-stimulated RAW 264.7 cells. Moreover, the treatments suppressed cytokines (prostaglandin E2 [PGE2], interleukin [IL]-1β, and IL-6) and nitrite production.
CONCLUSIONS
Both Magnoliae Cortex and maize exerted an anti-inflammatory effect on P. gingivalis-stimulated RAW 264.7 cells, and this effect was more pronounced when the extracts were combined. These findings show that these extracts may be beneficial for slowing the progression of periodontal disease.

Keyword

Cytokines; Magnolia; Mitogen-activated protein kinase 3; Periodontal diseases; Porphyromonas gingivalis; Zea mays

MeSH Terms

Blotting, Western
Cytokines
Enzyme-Linked Immunosorbent Assay
Inflammation
Interleukins
Magnolia*
Mitogen-Activated Protein Kinase 3
Nitric Oxide Synthase Type II
Periodontal Diseases
Porphyromonas gingivalis
Porphyromonas*
Protein Kinases
RAW 264.7 Cells
Transcription Factors
Zea mays*
Cytokines
Interleukins
Mitogen-Activated Protein Kinase 3
Nitric Oxide Synthase Type II
Protein Kinases
Transcription Factors
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