J Korean Acad Periodontol.  2003 Dec;33(4):555-562. 10.5051/jkape.2003.33.4.555.

Reduced alveolar bone loss in rats immunized with Porphyromonas gingivalis heat shock protein

• Yi NN
• Lee JY
• Choi JI

• KMID: 1999777
• DOI: http://doi.org/10.5051/jkape.2003.33.4.555

Abstract

The present study has been performed to evaluate Porphyromonas gingivalis (P.gingivalis) heat shock protein(HSP)60 as a candidate vaccine to inhibit multiple bacteria-induced alveolar bone loss. Rats were immunized with P.gingivalis HSP60 and experimental alveolar bone loss was induced by infection with multiple periodonto -pathogenic bacteria. Post-immune rat anti-P.gingivalis HSP IgG levels were significantly elevated and have demonstrated highly significant inverse relationship with the amount of alveolar bone loss induced by multiple bacteria. Results from PCR detection of subgingival bacterial plaque indicated that the vaccine successfully eradicated the multiple pathogenic species. We concluded that P.gingivalis HSP60 could potentially be developed as a vaccine to inhibit periodontal disease induced by multiple pathogenic bacteria.

Keyword

Heat shock protein; Porphyromonas gingivalis; vaccine

MeSH Terms

Alveolar Bone Loss*
Animals
Bacteria
Heat-Shock Proteins*
Hot Temperature*
Immunoglobulin G
Periodontal Diseases
Polymerase Chain Reaction
Porphyromonas gingivalis*
Porphyromonas*
Rats*
Shock
Heat-Shock Proteins
Immunoglobulin G