J Korean Acad Periodontol.  2003 Jun;33(2):179-191. 10.5051/jkape.2003.33.2.179.

Epitope specificity of Porphyromonas gingivalis heat shock protein for T-cell and/or B-cell in human atherosclerosis

• Bak JG
• Kim SJ
• Choi JI

• KMID: 1999798
• DOI: http://doi.org/10.5051/jkape.2003.33.2.179

Abstract

Since periodontal infections are suggested as risk factors for the development of cardiovascular diseases, the present study was performed to evaluate the T cell immune responses specific to Porphylomonas gingivalis(P. gingivalis) heat shock protein(hsp) 60 and T-cell and B-cell epitope specificities for P. gingivalis hsp60 in atherosclerosis. Anti-P. gingivalis IgG antibody titers were elevated in all patients. We could establish P. gingivalis hsp-specific T cell lines from the atheroma lesions, a mixture of CD4+ and CD8+ cells producing the cytokines characteristic of both Th1 and Th2 subsets. of 108 overlapping synthetic peptides spanning whole P. gingivalis hsp60 molecule, ten peptides with common epitopes specificities for both T-cell and B-cell were identified. it was concluded that P. gingivalis hsp60 might be involved in the immunoregulatory process of atherosclerotic diseases with epitope specificities.

Keyword

Porphyromonas gingivalis heat shock protein; Epitope; Atherosclerosisz

MeSH Terms

Atherosclerosis*
B-Lymphocytes*
Cardiovascular Diseases
Cell Line
Cytokines
Epitopes
Epitopes, B-Lymphocyte
Heat-Shock Proteins*
Hot Temperature*
Humans*
Immunoglobulin G
Peptides
Plaque, Atherosclerotic
Porphyromonas gingivalis*
Porphyromonas*
Risk Factors
Sensitivity and Specificity*
Shock
T-Lymphocytes*
Cytokines
Epitopes
Epitopes, B-Lymphocyte
Heat-Shock Proteins
Immunoglobulin G
Peptides