Nat Prod Sci.  2018 Mar;24(1):36-39. 10.20307/nps.2018.24.1.36.

Content Analysis of Rutin in the Leaves of Boehmeria nivea Harvested in Different Regions of South Korea by HPLC-UV

• Cho S
• Quilantang NG
• Lee JS
• Kim YM
• Kim HB
• Cho EJ
• Jung YS
• Lee S

• KMID: 2409607
• DOI: http://doi.org/10.20307/nps.2018.24.1.36

Abstract

Phytochemical analysis of Boehmeria nivea (Bn) leaves by medium pressure liquid chromatography led to the isolation of a flavonoid glycoside identified by spectroscopic analysis as rutin. The amount of rutin in the leaves of Bn harvested from nine regions in South Korea (Bn 1-9) which were collected on the months of June, July, August, and September was determined by HPLC-UV analysis. A gradient elution program that utilizes a Discovery® C18 (4.6 × 250 mm, 5 µm) column and mobile phase composed of 1% acetic acid-water: acetonitrile (90:10 to 60:40 for min) was followed. The injection volume and flow rate were 10 µl and 1 mL/ min, respectively. UV detection was set at 350 nm. Results show that Bn-8 harvested in September reported the highest content of rutin among the samples analyzed. This study provides a basis for the optimal harvest time of Bn which maximizes the yield of rutin.

Keyword

Boehmeria nivea; HPLC-UV; Rutin; Content analysis

MeSH Terms

Boehmeria*
Chromatography, Liquid
Korea*
Rutin*
Rutin

Figure

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  Fig. 1. Structure of rutin.

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  Fig. 2. Calibration curve of rutin.

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  Fig. 3. HPLC chromatograms of rutin (A) and MeOH extract of B. nivea (B).

Reference

References

(1). Angelini L. G.., Lazzeri A.., Levita G.., Fontanelli D.., Bozzi C.Ind. Crops Prod. 2000. 11:145–161.

(2). Paiva Junior C. Z.., de Carvalho L. H.., Fonseca V. M.., Monteiro S. N.., d'Almeida J. R. M.Polym. Test. 2004. 23:131–135.

(3). Nam S.., Netravali A. N.Fibers Polym. 2006. 7:372–379.

(4). Xu Q. -M.., Liu Y. -L.., Li X. -R.., Li X.., Yang S.-L. Nat. Prod. Res. 2011. 25:640–647.

(5). Lin C. -C.., Yen M. -H.., Lo T. -S.., Linb J. -M. J.Ethnopharmacol. 1998. 60:9–17.

(6). Sancheti S.., Sancheti S.., Bafna M.., Kim H. -R.., You Y. -H.., Seo S. -Y.Braz. J. Pharm. 2011. 21:146–154.

(7). Sung M. J.., Davaatseren M.., Kim S. H.., Kim M. J.., Hwang J.-T. Pharm. Biol. 2013. 51:1131–1136.

(8). Tan Z.., Wang C.., Yi Y.., Wang H.., Li M.., Zhou W.., Tan S.., Li F.Sep. Purif. Technol. 2014. 132:396–400.

(9). Cho S.., Lee D. G.., Jung Y. -S.., Kim H. B.., Cho E. J.., Lee S.Nat. Prod. Sci. 2016. 22:134–139.

(10). Couch J. F.., Naghski J.., Krewson C. F. J.Am. Chem. Soc. 1952. 74:424–425.

(11). Ohsawa R.., Tsutsumi T.Euphytica. 1995. 86:183–189.

(12). Chua L. S. J.Ethnopharmacol. 2013. 150:805–817.

(13). Kraujalis P.., Venskutonis P. R.., Ibáñez E.., Herrero M. J.Supercrit. Fluids. 2015. 104:234–242.

(14). Erlund I.., Kosonen T.., Alfthan G.., Mäenpää J.., Perttunen K.., Kenraali J.., Parantainen J.., Aro A.Eur. J. Clin. Pharmacol. 2000. 56:545–553.

(15). Hosseinzadeh H.., Nassiri-Asl M. J.Endocrinol. Invest. 2014. 37:783–788.

(16). Kitabayashi H.., Ujihara A.., Hirose T.., Minami M.Breeding Sci. 1995. 45:189–194.

(17). Gevrenova R.., Kitanov G.., Ilieva D.Pharm. Biol. 2007. 45:149–155.