Korean J Anat.  2003 Feb;36(1):49-56.

The Effect of Rutin on the Melanogenesis and Nitric Oxide in UVB-irradiated HM3KO Human Melanoma

• Lee SY
• Kim YM
• Lee JK
• Yoo SJ
• Mun YJ
• Woo WH

Abstract

Ultraviolet (UV) radiation is the main physiological stimulus for human skin pigmentation. Recently, nitric oxide (NO) have been involved in mediation of skin pigmentation induced by UVB. Rutin, a flavonoid of vegetables and fruits, has antiviral and antioxidant properties. Therefore, we investigated the effect of rutin on UVB-induced melano-genesis and NO production in HM3KO cells. In this study, we demonstrated that UVB-irradiation stimulated melanin content and tyrosinase activity in HM3KO cells. Rutin suppressed UVB-stimulated total melanin content and tyrosinase activity in a dose-dependent manner. Additionally, we showed that UVB-irradiation stimulated NO production in HM3KO cells. Rutin also suppressed UVB-induced NO production and repaired reduction of cell proliferation by UVB. UVB stimulation of melanogenesis was mimicked by exogenous NO donor (sodium nitroprusside, SNP), and rutin effectively suppressed it. Therefore, we concluded that rutin suppressed UVB-stimulated melanogenesis and that it is involved in melanogenesis regulation partially through the suppression of UVB-induced NO production.

Keyword

Rutin; Melanin; Tyrosinase activity; UVB; Nitric oxide (NO)

MeSH Terms

Cell Proliferation
Fruit
Humans*
Melanins
Melanoma*
Monophenol Monooxygenase
Negotiating
Nitric Oxide*
Nitroprusside
Rutin*
Skin Pigmentation
Tissue Donors
Vegetables
Melanins
Monophenol Monooxygenase
Nitric Oxide
Nitroprusside
Rutin