Nat Prod Sci.  2016 Jun;22(2):134-139. 10.20307/nps.2016.22.2.134.

Phytochemical Identification from Boehmeria nivea Leaves and Analysis of (-)-Loliolide by HPLC

Affiliations
  • 1Department of Integrative Plant Science, Chung-Ang University, Anseong 17546, Korea. slee@cau.ac.kr
  • 2Yeong-Gwang Agricultural Technology Center, Yeonggwang 57031, Korea.
  • 3Life Sciences Research Institute, Biomedic Co., Ltd., Bucheon 14548, Korea.
  • 4Department of Food Science and Nutrition, Pusan National University, Busan 46241, Korea.

Abstract

Phytochemicals were isolated from leaves of the fiber crop, ramie (Boehmeria nivea, Bn), using open column chromatography and medium pressure liquid chromatography. Their structures were identified as β-sitosterol, (-)-loliolide, rutin, and pyrimidinedione by MS, ¹H-, and ¹³C-NMR spectroscopic analysis. Among them, (-)-loliolide was isolated for the first time from B. nivea. A content analysis of (-)-loliolide in B. nivea collected from different regions and harvest times was conducted by HPLC. The highest content of (-)-loliolide was found in Bn-23 harvested in September. These results will be helpful to use the plant which harvest in September as a high content phytochemical additive in food, health supplements, and medicinal products.

Keyword

Boehmeria nivea; Urticaceae; Phytochemical; Identification; Loliolide; NMR

MeSH Terms

Boehmeria*
Chromatography
Chromatography, High Pressure Liquid*
Chromatography, Liquid
Phytochemicals
Plants
Rutin
Urticaceae
Rutin
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