Kidney Res Clin Pract.  2018 Mar;37(1):30-40. 10.23876/j.krcp.2018.37.1.30.

Human CD36 overexpression in renal tubules accelerates the progression of renal diseases in a mouse model of folic acid-induced acute kidney injury

• Jung JH
• Choi JE
• Song JH
• Ahn SH

• KMID: 2409372
• DOI: http://doi.org/10.23876/j.krcp.2018.37.1.30

Abstract

BACKGROUND
Acute kidney injury (AKI) is a risk factor for progression to chronic kidney disease, with even subclinical AKI episodes progressing to chronic kidney disease. Several risk factors such as preexisting kidney disease, hyperglycemia, and hypertension may aggravate renal disease after AKI. However, mechanisms underlying the progression of AKI are still unclear. This study identified the effect of human cluster of differentiation 36 (CD36) overexpression on the progression of folic acid-induced AKI.
METHODS
Pax8-rtTA/tetracycline response element-human CD36 transgenic mice were used to elucidate the effect of human CD36 overexpression in the proximal tubules on folic acid-induced AKI.
RESULTS
Results of histological analysis showed severely dilated tubules with casts and albuminuria in folic acid-treated transgenic mice overexpressing human CD36 compared with folic acid-treated wild-type mice. In addition, analysis of mRNA expression showed a significant increase in the collagen 3a1 gene in folic acid-treated transgenic mice overexpressing human CD 36 compared with folic acid-treated wild type mice.
CONCLUSION
Human CD36-overexpressing transgenic mice showed severe pathological changes and albuminuria compared with wild-type mice. Moreover, mRNA expression of the collagen 3a1 gene increased in folic acid-treated transgenic mice. These results suggest that human CD36 overexpression is a risk factor of AKI and its progression to chronic kidney disease.

Keyword

Acute kidney injury; Fibrosis; Folic acid; Renal insufficiency

MeSH Terms

Acute Kidney Injury*
Albuminuria
Animals
Collagen
Fibrosis
Folic Acid
Humans*
Hyperglycemia
Hypertension
Kidney Diseases
Mice*
Mice, Transgenic
Renal Insufficiency
Renal Insufficiency, Chronic
Risk Factors
RNA, Messenger
Collagen
Folic Acid
RNA, Messenger