Clin Exp Reprod Med.
2018 Mar;45(1):1-9. 10.5653/cerm.2018.45.1.1.
Variable localization of Toll-like receptors in human fallopian tube epithelial cells
- Affiliations
-
- 1Department of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
- 2Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. R.Aflatoonian@gmail.com
- 3Department of Anatomy, Tehran University of Medical Sciences, Tehran, Iran.
- 4Department of Immunology, Iran University of Medical Sciences, Tehran, Iran.
- 5Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
- 6Academic Unit of Reproductive and Developmental Medicine, Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK.
- KMID: 2408570
- DOI: http://doi.org/10.5653/cerm.2018.45.1.1
Abstract
OBJECTIVE
To determine the localization, expression, and function of Toll-like receptors (TLRs) in fallopian tube epithelial cells.
METHODS
The localization of TLRs in fallopian tube epithelial cells was investigated by immunostaining. Surprisingly, the intensity of staining was not equal in the secretory and ciliated cells. After primary cell culture of fallopian tube epithelial cells, ring cloning was used to isolate colonies of ciliated epithelial cells, distinct from non-ciliated epithelial cells. The expression of TLRs 1-10 was examined by quantitative real-time polymerase chain reaction, and protein localization was confirmed by immunostaining. The function of the TLRs was determined by interleukin (IL)-6 and IL-8 production in response to TLR2, TLR3, TLR5, TLR7, and TLR9 ligands.
RESULTS
Fallopian tube epithelial cells expressed TLRs 1-10 in a cell-type-specific manner. Exposing fallopian tube epithelial cells to TLR2, TLR3, TLR5, TLR7, and TLR9 agonists induced the secretion of proinflammatory cytokines such as IL-6 and IL-8.
CONCLUSION
Our findings suggest that TLR expression in the fallopian tubes is cell-type-specific. According to our results, ciliated cells may play more effective role than non-ciliated cells in the innate immune defense of the fallopian tubes, and in interactions with gametes and embryos.
MeSH Terms
-
Clone Cells
Cloning, Organism
Cytokines
Embryonic Structures
Epithelial Cells*
Fallopian Tubes*
Female
Germ Cells
Humans*
Interleukin-6
Interleukin-8
Interleukins
Ligands
Primary Cell Culture
Real-Time Polymerase Chain Reaction
Toll-Like Receptors*
Cytokines
Interleukin-6
Interleukin-8
Interleukins
Ligands
Toll-Like Receptors
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