Infect Chemother.  2018 Mar;50(1):29-37. 10.3947/ic.2018.50.1.29.

Genotypic Diversity of Multidrug Resistant Shigella species from Iran

Affiliations
  • 1Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. rezaee@tbzmed.ac.ir
  • 2Department of Microbiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
  • 3Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
  • 4Iranian Social Security Organization, Emam Reza Hospital, Urmia, Iran.
  • 5Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
  • 6Department of Microbiology and Virology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.

Abstract

BACKGROUND
In many developing countries, shigellosis is endemic and also occurs in epidemics and treatment of multidrug-resistant (MDR) isolates are important. The aims of this study were to determine the antimicrobial susceptibility, prevalence of class 1 and 2 integrons and the clonal relatedness of isolates.
MATERIALS AND METHODS
Antimicrobial susceptibility tests were performed by disc diffusion method. Polymerase chain reaction (PCR)-sequencing technique was employed for detection and characterization of integrons. The genetic relatedness was evaluated by using enterobacterial repetitive intergenic consensus (ERIC) PCR.
RESULTS
There was a high percentage of resistance to trimethoprim-sulfamethoxazole (TMP/SMX) (93.7%), ampicillin (AMP) (87.3%), streptomycin (STR) (84.5%) and tetracycline (TET) (78.9%). Multidrug resistant phenotype was seen in 95.1% of total isolates. Most common MDR profile was TMP/SMX/STR/AMP resistant pattern. Among the 142 Shigella spp. analyzed in this study, 28 isolates were positive for class 1 integron with two types of gene cassette arrays (dfrA17/aadA5 = 31.7% and dfrA7 = 3.8%). The class 2 integron was more frequently detected among the isolates (94.7%) with dfrA1/sat1/aadA1 (69.4%) and dfrA1/sat1 (30.6%) gene cassettes. ERIC-PCR results showed 6, 5, 4 and 3 main genotypes among S. flexneri, S. sonnei, S. boydii and S. dysenteriae isolates, respectively.
CONCLUSIONS
Our findings revealed that multidrug resistant Shigella species with high prevalence of class 2 integron were very common in Iran. In addition, ERIC-PCR patterns showed limited variety of clones are responsible for shigellosis in the region of the study.

Keyword

Shigella; Integron; Multidrug resistant; Enterobacterial repetitive intergenic consensus; Polymerase chain reaction

MeSH Terms

Ampicillin
Clone Cells
Consensus
Developing Countries
Diffusion
Dysentery, Bacillary
Genotype
Integrons
Iran*
Methods
Phenotype
Polymerase Chain Reaction
Prevalence
Shigella*
Streptomycin
Tetracycline
Trimethoprim, Sulfamethoxazole Drug Combination
Ampicillin
Streptomycin
Tetracycline
Trimethoprim, Sulfamethoxazole Drug Combination
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