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Detection of Severe Acute Respiratory Syndrome Coronavirus 2 in the Pleural Fluid

Baek MS, Kim WY, Lee KJ, Noh CS

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can be detected via a nasopharyngeal swab and in sputum, blood, urine, and feces. However, there is only limited data on the real-time...
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Developing species-specific quantitative real-time polymerase chain reaction primers for detecting Lautropia mirabilis

Park SN, Kook JK

This study aimed to develop Lautropia mirabilis-specific quantitative real-time polymerase chain reaction (qPCR) primers based on the sequence of DNA-directed RNA polymerase subunit beta gene. The PrimerSelect program was used...
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Diagnostic Evaluation of the BioFire ® Meningitis/Encephalitis Panel: A Pilot Study Including Febrile Infants Younger than 90 Days

Kim KM, Park JY, Park KU, Sohn YJ, Choi YY, Han MS, Choi EH

Purpose: Rapid detection of etiologic organisms is crucial for initiating appropriate therapy in patients with central nervous system (CNS) infection. This study aimed to evaluate the diagnostic value of the...
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A Patient with Immunocompetent Cryptococcal Meningitis Showing Repetitive False-Negative Immunoassay for Cryptococcal Antigen

Jung M, Oh H, Kim Kw, Kim MJ, Koo YS

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Molecular detection and characterization of ovine herpesvirus-2 using heminested PCR in Pakistan

Riaz A, Dry I, Dalziel R, Rehman SU, Shah MA, Akhtar HMN, Yousaf A, Baig R

Background: Malignant catarrhal fever (MCF) is a highly fatal lymphoproliferative disease of cattle, deer, bison, water buffalo, and pigs caused by the gamma-herpesviruses alcelaphine herpesvirus-1 (AlHV-1) and ovine herpesvirus-2 (OvHV-2). Objectives:...
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Practical Diagnostic Approach to Myopathy

Lee JM

Hereditary myopathy is characterized by the weakness of skeletal muscles and is associated with various genetic defects. The efficiency of a genetic diagnosis has been archived with wide application of...
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Prevalence of Respiratory Viral Infection Using Multiplex Reverse Transcriptase-Polymerase Chain Reaction

Kim JS, Kim JM, Choi SK, Cho YJ, Kim SJ, Kim JA

Background: We wanted to understand the prevalence of respiratory viral infections in children hospitalized for acute respiratory infections stratified by sex, age, season, multiple infections, and clinical symptoms. Methods: Out of...
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Usefulness of Sputum and Pharyngeal Swab Specimens for Diagnosis of Coronavirus Disease 2019

Yun HS, Jang DS, Lee SH

Background: Various specimens are possible for the diagnosis of COVID-19, but previous studies comparing samples were small studies with hospitalized patients. The purpose of this study is to help diagnose...
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The Relationship of Leptin (+19) AG, Leptin (2548) GA, and Leptin Receptor Gln223Arg Gene Polymorphisms with Obesity and Metabolic Syndrome in Obese Children and Adolescents

Bilge S, Yılmaz R, Karaslan E, Özer S, Ateş , Ensari E, Demir O

Purpose: Obesity is defined as the abnormal or excessive accumulation of fat over acceptable limits. Leptin is a metabolic hormone present in the circulation in amounts proportional to fat mass....
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Pathologically and Genetically Diagnosed Subclinical Symptomatic Duchenne Muscular Dystrophy Carrier: Broadened Spectrum of Clinical Phenotype

Cho MS, Lee JM

A 29-year-old female presented with an elevated level of serum creatine kinase without subjective weakness. Neurologic examination showed the subtle motor weakness of the right arm. Muscle biopsy showed dystrophic...
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A comparative study of the prevalence of Helicobacter pylori in the oral biofilms of a group of dental and nondental undergraduates from Sri Lanka

Mallikaarachchi M, Rajapakse S, Gunawardhana K, Jayatilake J

Dental health care workers (DHCW) are at a risk of occupational exposure to Helicobacter pylori from the aerosolized oral biofilms and saliva of patients. We designed this study to investigate...
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Expression of neurotrophic factor genes by human adipose stem cells post-induction by deprenyl

Amiri A, Kashani MHG, Ghorbanian MT

Human adipose stem cells (hASCs) were introduced as appropriate candidate due to advantages like ease of isolation, in vitro expansion and lack of immune response. Deprenyl (Dep) was used to...
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Effectiveness of Cytochrome c Oxidase Subunit 1 Gene Nested Polymerase Chain Reaction Compared to Dermoscopy or Microscopy Alone for the Detection and Diagnosis of Sarcoptes scabiei var. hominis Infection

Yang SY, Kim SS, Kim CW

Background: While microscopy (MS) evaluation of skin scrapings has a 100% positive predictive value and specificity by definition for scabies diagnosis, it has low sensitivity. Dermoscopy (DS) has not yet...
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Clinical efficacy of respiratory virus detection by using the FilmArray method in children admitted with respiratory infection

Lee HJ, Park JH, Kim JM, Kim JH, Baek HS

Purpose: Respiratory virus infection is a common cause of hospitalization in children. Rapid testing for respiratory viruses, such as the FilmArray method, can be clinically useful. However, insufficient evidence exists...
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Correlation between goose circovirus and goose parvovirus with gosling feather loss disease and goose broke feather disease in southern Taiwan

Ting CH, Lin CY, Huang YC, Liu SS, Peng SY, Wang CW, Wu HY

Background: Goslings in several Taiwanese farms experienced gosling feather loss disease (GFL) at 21–35 days and goose broke feather disease (GBF) at 42–60 days. The prevalence ranges from a few...
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Laboratory Diagnosis of COVID-19 in Korea

Lee K

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the cause of coronavirus disease 2019 (COVID-19), is a type of human coronavirus that causes severe pneumonia, similar to SARS-CoV-1 and Middle East...
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The epidemiology of amoebiasis in Thi-Qar Province, Iraq (2015-2020): differentiation of Entamoeba histolytica and Entamoeba dispar using nested and real-time polymerase chain reaction

Flaih MH, Khazaal RM, Kadhim MK, Hussein KR, Alhamadani FAB

OBJECTIVES: The objective of this study was to evaluate the present status of amoebiasis in Thi-Qar Province in southern Iraq, and to determine the presence of Entamoeba histolytica and Entamoeba...
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Comparative analysis of serological tests and fecal detection in the diagnosis of Mycobacterium avium subspecies paratuberculosis infection

Park HT, Ha S, Park HE, Shim S, Hur TY, Yoo HS

Abstract: Johne's disease (JD) caused by Mycobacterium avium subspecies paratuberculosis (MAP) is a chronic, wasting infectious disease in ruminants that causes enormous economic losses to the dairy and beef cattle...
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Usefulness of Nested Polymerase Chain Reaction with Clinical Specimens for Diagnosis of Leptospirosis: a Case Series and a Review of Literature

Na YS, Kim HL, Kim CM, Yun NR, Kim DM

A culture of the Leptospira species and the microscopic agglutination test (MAT) are considered as the reference standard for the diagnosis of leptospirosis, but both tests are imperfect for early...
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Successful Treatment of Mycoplasma hominis Meningitis, Diagnosed Using Real-Time Polymerase Chain Reaction, with Ciprofloxacin in a Neonate

Gwon I, Song WS, Sohn YH, Kim SY

Mycoplasma hominis can cause life-threatening central nervous system infections in neonates following intrauterine infection or during delivery. In newborns, the dia gnosis and treatment of M. hominis meningitis are challenging,...
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