Korean J Otorhinolaryngol-Head Neck Surg.
2017 Aug;60(8):396-403. 10.3342/kjorl-hns.2017.17601.
High Concentration of Glucose Induces MUC5B Expression via Reactive Oxygen Species in Human Airway Epithelial Cells
- Affiliations
-
- 1Department of Otorhinolaryngology-Head and Neck Surgery, Yeungnam University, Daegu, Korea. ssykhs@med.yu.ac.kr
- 2Regional Center for Respiratory Diseases, Yeungnam University Medical Center, Daegu, Korea.
- 3Department of Internal Medicine, College of Medicine, Yeungnam University, Daegu, Korea.
- KMID: 2390897
- DOI: http://doi.org/10.3342/kjorl-hns.2017.17601
Abstract
- BACKGROUND AND OBJECTIVES
The effects of hyperglycemia on the mucin secretion in inflammatory respiratory diseases are not clear. Therefore, this study was conducted to characterize the effect of hyperglycemia, and the mechanism involved, on MUC5AC and MUC5B expression in human airway epithelial cells.
SUBJECTS AND METHOD
The NCI-H292 cells and the primary cultures of human nasal epithelial cells were exposed to different concentration of glucose (5, 10, 15, 20, 30 mM) for 8 or 24 hours, the effects of high concentration of glucose (20 mM) on MUC5AC and MUC5B expression were determined using reverse transcriptase-polymerase chain reaction (PCR), real-time PCR and enzyme immunoassay. Measurement of reactive oxygen species (ROS) production was performed by flow cytometry. To investigate the role of ROS in high concentration of glucose-induced MUC5B expression, the cells were pretreated with N-acetyl-cysteine (NAC, 50 mM) as a ROS scavenger, or diphenyleneiodonium (DPI, 100 nM) as a nicotinamide adenine dinucleotide phosphate oxidase inhibitor for 1 hour.
RESULTS
In the NCI-H292 cells and the primary cultures of human nasal epithelial cells, High concentration of glucose increased MUC5B expression but did not increase MUC5AC expression (p<0.05). ROS production was also increased by high concentration of glucose (20 mM) (p<0.05). In addition, high concentration of glucose (20 mM)-induced MUC5B expression and ROS production were significantly attenuated by pretreatment of NAC (50 mM) or DPI (100 nM) (p<0.05).
CONCLUSION
High concentration of glucose induces MUC5B expressions via ROS in human airway epithelial cells.
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