J Korean Ophthalmol Soc.  2006 May;47(5):828-833.

The Effects of Glucose Concentrations on Reactive Oxygen products in Human Lens Epithelial Cells

• Yang YR
• Lee BR
• Koh JW

Abstract

PURPOSE: To investigate the effects of glucose concentrations on the formation of reactive oxygen products and cellular injury in human lens epithelial (HLE) cells.
METHODS
Human lens epithelial (HLE) cells were cultured with high glucose (200 mg/100 ml, 300 mg/100 ml, 400 mg/100 ml) and normal glucose (100 mg/100 ml) contained Modified Egle's Medium (MEM). The amounts of reactive oxygen products were assayed by dihydroethidium (DHE). Paraquat-induced cytotoxicity was determined by the 3-[4,5-dimethylthiazol-2-yl]-2,5 -diphenyl tetrazolium bromide (MTT) method.
RESULTS
Reactive oxygen products of human lens epithelial cells increased 110%, 250% and 630% in the high glucose (200 mg/100 ml, 300 mg/100 ml, 400 mg/100 ml) media compared to the normal glucose (100 mg/100 ml) media. The paraquat-induced cells toxicity was increased by the high glucose.
CONCLUSIONS
High glucose increased the formation of reactive oxygen products in human lens epithelial cells. These results suggest that high glucose can induce cataract formation in diabetic patients.

Keyword

High Glucose; Human Lens Epithelial Cells; Reactive Oxygen

MeSH Terms

Cataract
Epithelial Cells*
Glucose*
Humans*
Oxygen*
Glucose
Oxygen