Int J Stem Cells.  2017 May;10(1):103-113. 10.15283/ijsc16032.

Cavin-2 Functions as a Suppressive Regulator in TNF-induced Mesenchymal Stromal Cell Inflammation and Angiogenic Phenotypes

• Annabi B
• Zgheib A
• Annabi B

• KMID: 2384607
• DOI: http://doi.org/10.15283/ijsc16032

Abstract

Tumour necrosis factor (TNF)-α activation of mesenchymal stromal cells (MSC) enhances their tumour-suppressive properties and tumour-homing ability. The molecular actors involved are unknown. We found that TNF induced MSC migration and tubulogenesis which correlated with a dose-dependent increase in Cavin-1 and Cavin-3 transcript levels. TNF triggered cyclooxygenase (COX)-2 expression, whereas specific siRNA-mediated gene silencing of Cavin-2 resulted in an amplified COX-2 expression, tubulogenesis, and migratory response partially due to a rapid and sustained increase in NF-κB phosphorylation status. Our results highlight a suppressive role for the caveolar component Cavin-2 in the angiogenic and inflammatory regulation of TNF-activated MSC.

Keyword

Mesenchymal stromal cells; Cavin; TNF; Cancer; Angiogenesis; Inflammation

MeSH Terms

Gene Silencing
Inflammation*
Mesenchymal Stromal Cells*
Necrosis
Phenotype*
Phosphorylation
Prostaglandin-Endoperoxide Synthases
Prostaglandin-Endoperoxide Synthases