J Korean Pediatr Soc.  1996 Feb;39(2):255-264.

The Effect of IFN- and PGE2 in the Production of TNF- by Human PBMC Stimulated with LPS

• Hahn YS
• Kim JG

Abstract

PURPOSE
The purpose of this study was to observe the effect of IFN- and PGE2 on TNF- production by human peripheral mononuclear cells(PBMC) that were stimulated with LPS.
METHODS
PBMC were separated by Ficoll-Hypaque gradient centrifugation from human peripheral venous blood and were incubated for 72 hours with or without LPS, IFN- , PGE2, and indomethacin according to various conditions. TNF- activity of PBMC culture supernatants was assayed by determining the cytotoxicity against L929 cells.
RESULTS
1) The production of TNF- by PBMC in response to LPS reached the highest level between 8-24 hours and returned to control level by 72 hours. 2) When IFN- was added together with LPS, LPS-induced TNF- production was enhanced and prolonged, which was more remarkable when IFN- was added at higher concentration or earlier than LPS. 3) When PGE2 was added together with LPS, LPS-induced TNF- production was suppressed. 4) The addition of IFN-gamma reduced the suppressive effect of PGE2 on LPS-induced TNF- production. 5) Enough amount of indomethacin enhanced production of TNF- by LPS.
CONCLUSIONS
IFN-gamma increased and PGE2 decreased the production of TNF- by PBMC which were stimulated with LPS. And PBMC which were pretreated with IFN-gamma were resistent to the suppressive effect of PGE2.

Keyword

TNF-gamma; LPS; IFN-gamma; PCE2

MeSH Terms

Centrifugation
Dinoprostone*
Humans*
Indomethacin
Dinoprostone
Indomethacin