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Ritalinic Acid Stimulates Human Sperm Motility and Maintains Vitality In Vitro

Harlev A, Henkel R, Samanta L, Agarwal A

PURPOSE: To evaluate the in vitro impact of ritalinic acid (RA), a major metabolite of methylphenidate (drug to treat attention-deficit hyperactivity disorder), on sperm motility, vitality and oxidative stress. MATERIALS AND...
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Specific tail swelling pattern in hypo-osmotic solution as a predictor of DNA fragmentation status in human spermatozoa

Kim SW, Nho EJ, Lee JY, Jee BC

OBJECTIVE: The aim of this study was to investigate DNA fragmentation status in human spermatozoa according to specific tail swelling patterns determined via hypo-osmotic swelling test (HOST). METHODS: Frozen semen samples...
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Evaluation of BacT/Alert FAN Plus Bottles for the Culture of Peritoneal Dialysate

Park MS, Yoo IY, Kang OK, Lee JE, Kim DJ, Huh HJ, Lee NY

BACKGROUND: A major complication of peritoneal dialysis (PD) is peritonitis, and bacterial culture of PD effluent in a blood culture bottle is the preferred technique for diagnosis of peritonitis. In...
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Reduction in post extraction waiting period for dental implant patients using plasma rich in growth factors: an in vivo study using cone-beam computed tomography

Arya V, Malhotra V, Rao JD, Kirti S, Malhotra S, Sharma RS

OBJECTIVES: This study examined the effects of plasma-rich growth factors (PRGF) on accelerating bone regeneration/repair in fresh extraction sockets, and determined the quality and quantity of bone by assessing the...
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An experimental investigation of the effects of chronic stress on bone-to-implant contact

Dundar S, Bozoglan A, Yaman F, Kirtay M, Bulmus O, Sahin Aydinyurt H, Solmaz MY, Yanen C

OBJECTIVES: This study aimed to investigate the effects of chronic restraint stress on the osseointegration of titanium implants. MATERIALS AND METHODS: Twenty adult male Wistar albino rats were used in the...
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Effects of Pre-analytical Variables on Cell-free DNA Extraction for Liquid Biopsy

Jeong TD, Kim MH, Park S, Chung HS, Lee JW, Chang JH, Huh J

BACKGROUND: Extraction of cell-free DNA (cfDNA) is a key step for determining the quality of cfDNA-related molecular diagnostics. We evaluated the effect of sample containers and sample storage conditions on...
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Evaluation of normal morphology, DNA fragmentation, and hyaluronic acid binding ability of human spermatozoa after using four different commercial media for density gradient centrifugation

Lee D, Jee BC

OBJECTIVE: Density gradient centrifugation (DGC) is frequently used to isolate high-motility fractions of spermatozoa. We compared the efficacy of four DGC media in terms of the percentage of morphologically normal...
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Removing Lipemia in Serum/Plasma Samples: A Multicenter Study

Castro-Castro MJ, Candás-Estébanez B, Esteban-Salán M, Calmarza P, Arrobas-Velilla T, Romero-Román C, Pocoví-Mieras M, Aguilar-Doreste JA, on behalf of the Commission on Lipoprotein and Vascular Diseases, Sociedad Española de Química Clínica

BACKGROUND: Lipemia, a significant source of analytical errors in clinical laboratory settings, should be removed prior to measuring biochemical parameters. We investigated whether lipemia in serum/plasma samples can be removed...
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Factors Affecting Hemagglutinations Strength in ABO Blood Group Typing Test Using the Tube Method

Jeon YL, Lee WI, Kang SY, Kim MH

BACKGROUND: The ABO blood group typing test (ABO test) is an initial pre-transfusion test based on hemagglutination. Although various factors affect hemagglutination strength, few studies have examined how these factors...
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Clostridium botulinum spores in Polish honey samples

Grenda T, Grabczak M, Sieradzki , Kwiatek K, Pohorecka , Skubida M, Bober A

The aim of this study was an examination of 240 multifloral honey samples collected from Polish apiaries to determine Clostridium botulinum occurrence. Honey was collected from apiaries directly after the...
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Non-cultured Epidermal Cell Suspension Transplantation Using Suction Blisters to Treat Refractory Vitiligo: A Retrospective Study of 20 Cases

Bae JM, Jung HM, Lee HN, Lee RW, Eun SH, Kwon HS, Lee JH, Kim GM

  • KMID: 2436845
  • Korean J Dermatol.
  • 2018 Aug;56(7):426-432.
BACKGROUND: As nonsurgical interventions for vitiligo are not always successful, various surgical modalities have been used in patients with refractory vitiligo. Of these, non-cultured epidermal suspension transplantation (NCES) was recently...
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Sperm DNA fragmentation and sex chromosome aneuploidy after swim-up versus density gradient centrifugation

Kim SW, Jee BC, Kim SK, Kim SH

OBJECTIVE: The aim of this study was to compare the efficacy of swim-up and density gradient centrifugation (DGC) for reducing the amount of sperm with fragmented DNA, sex chromosome aneuploidy,...
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Necessity of purification during bacterial DNA extraction with environmental soils

Lim HJ, Choi JH, Son A

Complexity and heterogeneity of soil samples have often implied the inclusion of purification steps in conventional DNA extraction for polymerase chain reaction (PCR) assays. Unfortunately the purification steps are also...
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Efficient isolation of sperm with high DNA integrity and stable chromatin packaging by a combination of density-gradient centrifugation and magnetic-activated cell sorting

Chi HJ, Kwak SJ, Kim SG, Kim YY, Park JY, Yoo CS, Park IH, Sun HG, Kim JW, Lee KH

OBJECTIVE: This study was carried out to investigate the correlations of the sperm DNA fragmentation index (DFI) with semen parameters and apoptosis, and to investigate the effects of density-gradient centrifugation...
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Ginsenoside Re Enriched Fraction (GS-F3K1) from Ginseng Berries Ameliorates Ethanol-Induced Erectile Dysfunction via Nitric Oxide-cGMP Pathway

Pyo MK, Park KH, Oh MH, Lee H, Park YS, Kim NY, Park SH, Song JH, Park JD, Jung SH, Lee BG, Won BY, Shin KY, Lee HG

Erectile dysfunction (ED) is a highly prevalent disorder that affects millions of men and considered to be an early symptom of atherosclerosis and a precursor of various systemic vascular disorders....
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16S rRNA gene-based metagenomic analysis reveals differences in bacteria-derived extracellular vesicles in the urine of pregnant and non-pregnant women

Yoo JY, Rho M, You YA, Kwon EJ, Kim MH, Kym S, Jee YK, Kim YK, Kim YJ

Recent evidence has indicated that bacteria-derived extracellular vesicles (EVs) are important for host–microbe communication. The aims of the present study were to evaluate whether bacteria-derived EVs are excreted via the...
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In Vitro Performance of Injectable Chitosan-Tripolyphosphate Scaffolds Combined with Platelet-Rich Plasma

Shimojo AA, Galdames S, Perez AG, Ito TH, Malheiros Luzo AC, Santana MH

This study aimed to evaluate the in vitro biological effectiveness of chitosan microparticles crosslinked with sodium tripolyphosphate (TPP) in combination with activated pure platelet-rich plasma (aP-PRP) as an injectable composite...
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Comparison of the Effectiveness between Sampling Methods for Protein Analysis of Tear Fluids

Lee CH, Yeo A, Kim TI, Seo KY, Kim EK, Lee HK

PURPOSE: Although various sampling methods of tears from conjunctival sac have been reported, no previous study compared their effectiveness or efficiency based on protein extraction. By comparing the compliance, volume...
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Protective effects of Artemisia arborescens essential oil on oestroprogestative treatment induced hepatotoxicity

Dhibi S, Ettaya A, Elfeki A, Hfaiedh N

BACKGROUND: Currently, natural products have been shown to exhibit interesting biological and pharmacological activities and are used as chemotherapeutic agents. The purpose of this study, conducted on Wistar rats, was...
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Esterase Activity and Intracellular Localization in Reconstructed Human Epidermal Cultured Skin Models

Tokudome Y, Katayanagi M, Hashimoto

BACKGROUND: Reconstructed human epidermal culture skin models have been developed for cosmetic and pharmaceutical research. OBJECTIVE: This study evaluated the total and carboxyl esterase activities (i.e., K(m) and V(max), respectively) and...
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