J Korean Soc Plast Reconstr Surg.  2006 Jul;33(4):423-426.

Proper Condition of Centrifugation for the Fat Cell Viability in the Autologous Fat Injection

• Jang KM
• Kim JY
• Yang JD
• Chung HY
• Park JW
• Cho BC

Abstract

PURPOSE
In the autologous fat injection, the centrifugation is useful for the refinement of harvested fat. As it can be an injury to the fat cell, we studied the fat cell viability with the change of centrifugation velocity and centrifugation time in order to get the limits of centrifugation velocity and centrifugation time.
METHODS
We used the Colman System in 8 patients. We handled the control group with no centrifugation, group I with the centrifugation with 1500rpm for 1 minute, group II with 1500 rpm for 3 minutes, group III with 1500rpm for 5 minutes, group IV with 3000rpm for 1 minute, group V with 3000rpm for 3 minutes, group VI with 3000rpm for 5 minutes, group VII with 5000rpm for 1 minute, group VIII with 5000rpm for 3 minutes, group IX with 5000rpm for 5 minutes. We used the collagenase to separate the fat tissue. We had evaluated the fat cell viability by checking survival cell counts.
RESULTS
There was no significance in group I, II, IV, V, but there was significant difference in group III, VI, VII, VIII, IX.
CONCLUSION
The centrifugation with 3000rpm for 3 minutes is recommendable.

Keyword

Fat cell viability; Centrifugation

MeSH Terms

Adipocytes*
Cell Count
Centrifugation*
Collagenases
Humans
Collagenases