Biomol Ther.  2015 Jul;23(4):333-338. 10.4062/biomolther.2015.062.

Chitin from Cuttlebone Activates Inflammatory Cells to Enhance the Cell Migration

• Lim SC
• Lee KM
• Kang TJ

• KMID: 2382362
• DOI: http://doi.org/10.4062/biomolther.2015.062

Abstract

Our previous report showed that the extract from cuttlebone (CB) had wound healing effect in burned lesion of rat and the extract was identified as chitin by HPLS analysis. We herein investigated the morphology in CB extract using scanning electron microscope (SEM). Chitin was used as a control. There is no difference in morphology between CB extract and chitin. We also assessed the role of CB extract on the production of inflammatory mediators using murine macrophages and the migration of inflammatory cells. The extract induced the production of nitric oxide (NO) in macrophages. While the extract of CB itself stimulated macrophages to increase the expression of pro-inflammatory cytokines such as tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, and IL-6, CB extract suppressed the production of those cytokines by LPS. CB extract also induced the production of mouse IL-8 which is related to the cell migration, and treatment with CB enhanced fibroblast migration and invasion. Therefore, our results suggest that CB activates inflammatory cells to enhance the cell migration.

Keyword

Cuttlebone; Macrophages; Inflammatory mediator; Fibroblast; Migration

MeSH Terms

Animals
Burns
Cell Movement*
Chitin*
Cytokines
Fibroblasts
Interleukin-6
Interleukin-8
Interleukins
Macrophages
Mice
Nitric Oxide
Rats
Tumor Necrosis Factor-alpha
Wound Healing
Chitin
Cytokines
Interleukin-6
Interleukin-8
Interleukins
Nitric Oxide
Tumor Necrosis Factor-alpha