Int J Stem Cells.  2015 May;8(1):99-105. 10.15283/ijsc.2015.8.1.99.

Combination Cell Therapy with Mesenchymal Stem Cells and Neural Stem Cells for Brain Stroke in Rats

• Hosseini S
• Farahmandnia M
• Razi Z
• Delavari S
• Shakibajahromi B
• Sarvestani FS
• Kazemi S
• Semsar M

• KMID: 2380803
• DOI: http://doi.org/10.15283/ijsc.2015.8.1.99

Abstract

OBJECTIVES
Brain stroke is the second most important events that lead to disability and morbidity these days. Although, stroke is important, there is no treatment for curing this problem. Nowadays, cell therapy has opened a new window for treating central nervous system disease. In some previous studies the Mesenchymal stem cells and neural stem cells. In this study, we have designed an experiment to assess the combination cell therapy (Mesenchymal and Neural stem cells) effects on brain stroke. METHOD AND MATERIALS: The Mesenchymal stem cells were isolated from adult rat bone marrow and the neural stem cells were isolated from ganglion eminence of rat embryo 14 days. The Mesenchymal stem cells were injected 1 day after middle cerebral artery occlusion (MCAO) and the neural stem cells transplanted 7 day after MCAO. After 28 days, the neurological outcomes and brain lesion volumes were evaluated. Also, the activity of Caspase 3 was assessed in different groups. RESULT: The group which received combination cell therapy had better neurological examination and less brain lesion. Also the combination cell therapy group had the least Caspase 3 activity among the groups.
CONCLUSIONS
The combination cell therapy is more effective than Mesenchymal stem cell therapy and neural stem cell therapy separately in treating the brain stroke in rats.

Keyword

Mesenchymal stem cells; Neural stem cells; Combination cell therapy; Brain stroke

MeSH Terms

Adult
Animals
Bone Marrow
Brain*
Caspase 3
Cell- and Tissue-Based Therapy*
Central Nervous System
Embryonic Structures
Ganglion Cysts
Humans
Infarction, Middle Cerebral Artery
Mesenchymal Stromal Cells*
Neural Stem Cells*
Neurologic Examination
Rats*
Stroke*
Caspase 3

Figure

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  Fig. 1 Rat bone marrow mesenchymal stem cells.

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  Fig. 2 Immunocytochemistry for CD44, CD45, CD90 and CD34.

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  Fig. 3 Neurospheres.

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  Fig. 4 Immunocytochemistry for β-tubulin and GFAP.

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  Fig. 5 Neurological function assessment.

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  Fig. 6 Histology study.

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  Fig. 7 Caspase 3 Activity Assay.

Reference

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