Int J Stem Cells.  2015 May;8(1):69-78. 10.15283/ijsc.2015.8.1.69.

Bone Marrow Mesenchymal Stem Cell Transplantation in a Rabbit Corneal Alkali Burn Model (A Histological and Immune Histo-chemical Study)

Affiliations
  • 1Department of Histology, Faculty of Medicine, Ain Shams University, Cairo, Egypt. saharhistology@gmail.com

Abstract

BACKGROUND
Alkali-burned corneas can seldom heal properly to restore corneal transparency. Treatment of this severe disorder of the ocular surface remains a challenge. AIM OF THE WORK: was to investigate whether systemically transplanted bone marrow mesenchymal stem cells (BM-MSCs) can promote corneal wound healing after alkali burn. MATERIAL AND METHODS: Thirty five male New Zealand rabbits were used in this study. The animals were divided into three groups. Group I; the control group was sham operated. Group II; corneal alkali burn was created. Group III; underwent corneal alkali burn then treated with BM-MSCs. All corneas were collected after fourteen and twenty eight days. Evaluation using H&E, PAS & alkaline phosphatase reaction was carried out. Immune histo-chemical staining for CD44 and vimentin was performed as well.
RESULTS
the corneal epithelium of (Group II) showed marked alterations. Vascularization, cellular infiltration and irregularity of the collagen fibers were also seen in the substantia propria. Increase in the thickness of the Descemet's membrane was noticed as well. On the other hand, at the time of 28 days, Group III rabbits showed best histological results with nearly healed corneas compared to other groups. Meanwhile, vimentin was more strongly expressed in Group III assessing the differentiating ability of BM-MSCs.
CONCLUSION
BM-MSCs could effectively promote corneal alkali burn healing.

Keyword

Corneal wound healing; Mesenchymal stem cell; Alkaline phosphatase; Vimentin

MeSH Terms

Alkalies*
Alkaline Phosphatase
Animals
Bone Marrow*
Burns*
Collagen
Cornea
Descemet Membrane
Epithelium, Corneal
Hand
Humans
Male
Mesenchymal Stem Cell Transplantation*
Mesenchymal Stromal Cells
Rabbits
Vimentin
Wound Healing
Alkalies
Alkaline Phosphatase
Collagen
Vimentin

Figure

  • Fig. 1 (A) BM-MSCs, on day five of isolation and culture, showing the appearance of a colony of star-shaped cells with long processes (↑), central vesicular nucleus and granular cytoplasm. Notice, a cell appears in the anaphase stage of division (*). Characterization of MSCs using CD44+ was evident as well. (B) Showing the usual layers of the cornea. The non-keratinized stratified squamous corneal epithelium (*) appears with basal columnar cells (B), intermediate polygonal cells (I) and flat surface cells (F). Thick stroma (S) containing regularly arranged fibers with sparsely distributed keratocytes (↑), homogenous Descemet’s membrane is seen (D). Notice, the simple squamous corneal endothelium (↓↓). (C) Showing PAS positive regular Descemet’s membrane (↑). (D) Showing weak alkaline phosphatase positive reaction in few corneal stromal cells (↓). (E) Showing negative immune-reaction for CD44 in the cytoplasm of epithelial and stromal cells. (F) Showing weak positive immune-reaction for vimentin in few substantia propria cells (↓). A=phase contrast inverted microscopy×200. Control group; B=H&E×250, C=PAS×560, D=Alkaline phosphatase reaction×250, E=Avidin–biotin peroxidase for CD44×250, F=Avidin–biotin peroxidase for vimentin×250.

  • Fig. 2 (A) Showing an area of discontinuation of the corneal epithelium and underlying substantia propria (↓). Notice, the formation of granulation tissue with appearance of blood vessels in the stroma (↓↓) together with mononuclear cellular infiltration (*). (B) Showing disorganization and hyperplasia of the corneal epithelial cells. Notice, the loss of demarcation between the epithelium and the underlying substantia propria (↑) together with absence of flat superficial epithelial cells. (C) Showing an apparent increase in the thickness of the wavy, irregular PAS positive Descemet’s membrane (↓). Notice, an increase in PAS positive reaction in some perpendicularly oriented fibrils of corneal stroma (*). (D) Showing few alkaline phosphatase positive stromal cells (↓). (E) Showing negative immune expression for CD44 in both epithelial and stromal cells. (F) Showing positive immue-reaction in few stromal cells in the anterior part of the substantia propria (↑). Subgroup IIa ; A=H&E×250, B=H&E×560, C=PAS×560, D=Alkaline phosphatase reaction×250, E=Avidin–biotin peroxidase for CD44×250, F=Avidin–biotin peroxidase for vimentin×250.

  • Fig. 3 (A) Showing discontinuation of the corneal epithelium (↓). Notice, the substantia propria with heavy mononuclear cellular infiltration (↓↓). The inset shows an apparent increase in thickness of corneal epithelium. The cells are overcrowded, some of the epithelial cells appear with clear cytoplasm and deeply stained nuclei (*). Sloughing of the superficial flat cells is obvious. Notice, the eosinophil with their acidophilic cytoplasm and bilobed nucleus in the underlying stroma (↑). (B) Showing the collagen fibers of substantia propria widely separated and irregularly oriented. An apparent increase in the thickness of Descemet’s membrane (D). Both substantia propria and Descemet’s membrane are heavily infiltrated by fibroblast (↓↓). the inset shows, Descemet’s membrane infiltration by branched basophilic fibroblast (→). (C) Showing an apparent thickening of PAS positive Descemet’s membrane as compared to control group (→). (D) Showing moderate alkaline phosphatase activity in some stromal cells (↓). (E) Showing negative immune expression for CD44 in both epithelial and stromal cells. (F) Showing few positive vimentin stromal cells in anterior part of substantia propria (↓). Subgroup IIb ; A=H&E×250 and inset×560, B=H&E×250 and inset×560, C=PAS×560, D=Alkaline phosphatase reaction ×250, E=Avidin–biotin peroxidase for CD44×250, F=Avidin–biotin peroxidase for vimentin×250.

  • Fig. 4 (A) Showing. some epithelial cells with clear cytoplasm and deeply stained nuclei (↑). (B) Showing, regular arrangement of Descemet’s membrane together with an apparent decrease in its thickness compared to subgroup IIIa (↑). (C) Showing, dense alkaline phosphatase positive cells in stroma (↓). Notice, the increase in the density of their reaction. (D) Showing, positive expression of CD44 in some basal epithelial cells (↑) as well as in stromal cells (↑↑). (E) Showing, positive expression of vimentin in most of stromal cells (↓). Subgroup IIIa ; A=H&E×560, B=PAS×560, C=Alkaline phosphatase reaction ×250, D=Avidin–biotin peroxidase for CD44×560, E=Avidin–biotin peroxidase for vimentin×250.

  • Fig. 5 (A) Showing the epithelial cells is formed of basal layer (↑), intermediate polygonal cells (*) and superficial flat cells (↓↓). Notice, regular arrangement of the collagen fibers in the stroma (c). (B) Showing the thickness of the Descemet’s membrane (↓) nearly similar to control group. (C) Showing an apparent increase in the number of alkaline phosphatase positive cells in the substantia propria (↓). (D) Showing brown cytoplasmic positive reaction of CD44 in some superficial epithelial cells (↓). (E) Showing an apparent increase in the number of vimentin positive cells in stroma. Notice the strong intensity of their reaction (↓). Subgroup IIIb ; A=H&E×560, B=PAS×560, C=Alkaline phosphatase reaction×250, D=Avidin–biotin peroxidase for CD44×560, E=Avidin–biotin peroxidase for vimentin×250.


Reference

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