Ewha Med J.  2017 Apr;40(2):77-86. 10.12771/emj.2017.40.2.77.

Role of β₁-Integrin in Colorectal Cancer: Case-Control Study

Affiliations
  • 1Department of Surgery, Ewha Womans University School of Medicine, Seoul, Korea. ralee@ewha.ac.kr

Abstract


OBJECTIVES
In the metastatic process, interactions between circulating tumor cells (CTCs) and the extracellular matrix or surrounding cells are required. β1-integrin may mediate these interactions. The aim of this study was to investigate whether β1-integrin is associated with the detection of CTCs in colorectal cancer.
METHODS
We enrolled 30 patients with colorectal cancer (experimental group) and 30 patients with benign diseases (control group). Blood samples were obtained from each group, carcinoembryonic antigen (CEA) mRNA for CTCs marker and β1-integrin mRNA levels were estimated by using reverse transcription-polymerase chain reaction, and the results were compared between the two groups.
RESULTS
CEA mRNA was detected more frequently in colorectal cancer patients than in control patients (P=0.008). CEA mRNA was significantly reduced after surgery in the colorectal cancer patients (P=0.032). β1-integrin mRNA was detected more in colorectal cancer patients than in the patients with benign diseases (P<0.001). In colorectal cancer patients, expression of β1-integrin mRNA was detected more for advanced-stage cancer than for early-stage cancer (P=0.033) and was significantly decreased after surgery (P<0.001). In addition, expression of β1-integrin mRNA was significantly associated with that of CEA mRNA in colorectal cancer patients (P=0.001).
CONCLUSION
In conclusion, β1-integrin is a potential prognostic factor following surgical resection in colorectal cancer patients. β1-integrin may be a candidate for use as a marker for early detection of micrometastatic tumor cells and for monitoring the therapeutic response in colorectal cancer patients.

Keyword

Integrins; Circulating neoplastic cells; Carcinoembryonic antigen; Colorectal neoplasms

MeSH Terms

Carcinoembryonic Antigen
Case-Control Studies*
Colorectal Neoplasms*
Extracellular Matrix
Humans
Integrins
Neoplastic Cells, Circulating
RNA, Messenger
Carcinoembryonic Antigen
Integrins
RNA, Messenger

Figure

  • Fig. 1 Detection of carcinoembryonic antigen (CEA) by real-time polymerase chain reaction (RT-PCR) in the blood of each group. CEA is detected more frequently in colorectal cancer patients as compared with the controls (P=0.008). In colorectal cancer patients, expression of CEA is significantly decreased after operation (P=0.032). β-actin is an internal control.

  • Fig. 2 Detection of β1-integrin by real-time polymerase chain reaction (RT-PCR) in the blood of each group. β1-integrin is detected more frequently in colorectal cancer patients as compared with the controls (P<0.001). In colorectal cancer patients, expression of β1-integrin is significantly decreased after operation (P<0.001). β-actin is an internal control.

  • Fig. 3 Correlation of β1-integrin & carcinoembryonic antigen (CEA). Expression of β1-integrin is significantly associated with that of CEA. (A) In control group, there is a moderate-positive correlation between β1-integrin and CEA (r=0.446, P=0.014). (B) In preoperative status of experimental group, there is a moderate positive correlation between β1-integrin and CEA (r=0.516, P=0.003). (C) In postoperative status of experimental group, there is a high-positive correlation between β1-integrin and CEA (r=0.935, P<0.001).


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