Ann Coloproctol.  2014 Apr;30(2):61-70. 10.3393/ac.2014.30.2.61.

Role of beta1-Integrin in Colorectal Cancer: Case-Control Study

• Oh BY
• Kim KH
• Chung SS
• Hong KS
• Lee RA

• KMID: 2263976
• DOI: http://doi.org/10.3393/ac.2014.30.2.61

Abstract

PURPOSE
In the metastatic process, interactions between circulating tumor cells (CTCs) and the extracellular matrix or surrounding cells are required. beta1-Integrin may mediate these interactions. The aim of this study was to investigate whether beta1-integrin is associated with the detection of CTCs in colorectal cancer.
METHODS
We enrolled 30 patients with colorectal cancer (experimental group) and 30 patients with benign diseases (control group). Blood samples were obtained from each group, carcinoembryonic antigen (CEA) mRNA for CTCs marker and beta1-integrin mRNA levels were estimated by using reverse transcription-polymerase chain reaction, and the results were compared between the two groups. In the experimental group, preoperative results were compared with postoperative results for each marker. In addition, we analyzed the correlation between the expressions of beta1-integrin and CEA.
RESULTS
CEA mRNA was detected more frequently in colorectal cancer patients than in control patients (P = 0.008). CEA mRNA was significantly reduced after surgery in the colorectal cancer patients (P = 0.032). beta1-Integrin mRNA was detected more in colorectal cancer patients than in the patients with benign diseases (P < 0.001). In colorectal cancer patients, expression of beta1-integrin mRNA was detected more for advanced-stage cancer than for early-stage cancer (P = 0.033) and was significantly decreased after surgery (P < 0.001). In addition, expression of beta1-integrin mRNA was significantly associated with that of CEA mRNA in colorectal cancer patients (P = 0.001).
CONCLUSION
In conclusion, beta1-integrin is a potential factor for forming a prognosis following surgical resection in colorectal cancer patients. beta1-Integrin may be a candidate for use as a marker for early detection of micrometastatic tumor cells and for monitoring the therapeutic response in colorectal cancer patients.

Keyword

beta1-Integrin; Circulating tumor cells; Carcinoembryonic antigen; Colorectal cancer

MeSH Terms

Carcinoembryonic Antigen
Case-Control Studies*
Colorectal Neoplasms*
Extracellular Matrix
Humans
Neoplastic Cells, Circulating
Prognosis
RNA, Messenger
Carcinoembryonic Antigen
RNA, Messenger