Biomol Ther.  2017 May;25(3):266-271. 10.4062/biomolther.2016.116.

Synthetic Cannabinoid-Induced Immunosuppression Augments Cerebellar Dysfunction in Tetanus-Toxin Treated Mice

• Yun J
• Gu SM
• Lee TH
• Song YJ
• Seong S
• Kim YH
• Cha HJ
• Han KM
• Shin J
• Oh H
• Jung K
• Ahn C
• Park HK
• Kim HS

• KMID: 2378804
• DOI: http://doi.org/10.4062/biomolther.2016.116

Abstract

Synthetic cannabinoids are one of most abused new psychoactive substances. The recreational use of abused drug has aroused serious concerns about the consequences of these drugs on infection. However, the effects of synthetic cannabinoid on resistance to tetanus toxin are not fully understood yet. In the present study, we aimed to determine if the administration of synthetic cannabinoids increase the susceptibility to tetanus toxin-induced motor behavioral deficit and functional changes in cerebellar neurons in mice. Furthermore, we measured T lymphocytes marker levels, such as CD8 and CD4 which against tetanus toxin. JWH-210 administration decreased expression levels of T cell activators including cluster of differentiation (CD) 3ε, CD3γ, CD74p31, and CD74p41. In addition, we demonstrated that JWH-210 induced motor impairment and decrement of vesicle-associated membrane proteins 2 levels in the cerebellum of mice treated with tetanus toxin. Furthermore, cerebellar glutamatergic neuronal homeostasis was hampered by JWH-210 administration, as evidenced by increased glutamate concentration levels in the cerebellum. These results suggest that JWH-210 may increase the vulnerability to tetanus toxin via the regulation of immune function.

Keyword

New psychoactive substances; Cytokine; T cell activator; Tetanus toxin; Motor impairment; Glutamate

MeSH Terms

Animals
Cannabinoids
Cerebellar Diseases*
Cerebellum
Glutamic Acid
Homeostasis
Immunosuppression*
Mice*
Neurons
R-SNARE Proteins
T-Lymphocytes
Tetanus
Tetanus Toxin
Cannabinoids
Glutamic Acid
R-SNARE Proteins
Tetanus Toxin