Biomol Ther.  2017 May;25(3):249-258. 10.4062/biomolther.2016.058.

Biflorin Ameliorates Memory Impairments Induced by Cholinergic Blockade in Mice

• Jeon SJ
• Kim B
• Ryu B
• Kim E
• Lee S
• Jang DS
• Ryu JH

• KMID: 2378802
• DOI: http://doi.org/10.4062/biomolther.2016.058

Abstract

To examine the effect of biflorin, a component of Syzygium aromaticum, on memory deficit, we introduced a scopolamine-induced cognitive deficit mouse model. A single administration of biflorin increased latency time in the passive avoidance task, ameliorated alternation behavior in the Y-maze, and increased exploration time in the Morris water maze task, indicating the improvement of cognitive behaviors against cholinergic dysfunction. The biflorin-induced reverse of latency in the scopolamine-treated group was attenuated by MK-801, an NMDA receptor antagonist. Biflorin also enhanced cognitive function in a naïve mouse model. To understand the mechanism of biflorin for memory amelioration, we performed Western blot. Biflorin increased the activation of protein kinase C-ζ and its downstream signaling molecules in the hippocampus. These results suggest that biflorin ameliorates drug-induced memory impairment by modulation of protein kinase C-ζ signaling in mice, implying that biflorin could function as a possible therapeutic agent for the treatment of cognitive problems.

Keyword

Biflorin; N-methyl D-aspartate receptor; Cognition; Protein kinase C-ζ

MeSH Terms

Animals
Blotting, Western
Cognition
Cognition Disorders
Dizocilpine Maleate
Hippocampus
Memory Disorders
Memory*
Mice*
N-Methylaspartate
Protein Kinases
Syzygium
Water
Dizocilpine Maleate
N-Methylaspartate
Protein Kinases
Water