Yonsei Med J.  2016 May;57(3):557-564. 10.3349/ymj.2016.57.3.557.

Isoprenaline Induces Periostin Expression in Gastric Cancer

Affiliations
  • 1Department of General Surgery, Chinese PLA General Hospital, Beijing, China. weibo@vip.163.com, 13381326128@163.com
  • 2Wound Healing and Cell Biology Laboratory, Institute of Basic Medical Science, Trauma Center of Postgraduate Medical School, Chinese PLA General Hospital, Beijing, China.
  • 3Department of Pathology, Chengde Medical College, Chengde, Hebei Province, China.

Abstract

PURPOSE
Periostin mediates critical steps in gastric cancer and is involved in various signaling pathways. However, the roles of periostin in promoting gastric cancer metastasis are not clear. The aim of this study was to investigate the relevance between periostin expression and gastric cancer progression and the role of stress-related hormones in the regulation of cancer development and progression.
MATERIALS AND METHODS
Normal, cancerous and metastatic gastric tissues were collected from patients diagnosed with advanced gastric cancer. The in vivo expression of periostin was evaluated by in situ hybridization and immunofluorescent staining. Meanwhile, human gastric adenocarcinoma cell lines MKN-45 and BGC-803 were used to detect the in vitro expression of periostin by using quantitative real-time polymerase chain reaction (PCR) and western blotting.
RESULTS
Periostin is expressed in the stroma of the primary gastric tumors and metastases, but not in normal gastric tissue. In addition, we observed that periostin is located mainly in pericryptal fibroblasts, but not in the tumor cells, and strongly correlated to the expression of α-smooth muscle actin (SMA). Furthermore, the distribution patterns of periostin were broader as the clinical staging of tumors progressed. We also identified a role of stress-related signaling in promoting cancer development and progression, and found that isoprenaline upregulated expression levels of periostin in gastric cancer cells.
CONCLUSION
These findings suggest that the distribution pattern of periostin was broader as the clinical staging of the tumor progressed and found that isoprenaline upregulated expression levels of periostin in gastric cancer cells.

Keyword

Gastric cancer; stress; metastasis; periostin; extracellular matrix; nich

MeSH Terms

Adenocarcinoma/*metabolism/pathology
Adrenergic beta-Agonists/pharmacology
Aged
Blotting, Western
Cell Adhesion Molecules/drug effects/*metabolism
Cell Line, Tumor
Fibroblasts/*metabolism
Gene Expression Regulation, Neoplastic/*drug effects
Humans
Isoproterenol/*pharmacology
Male
Neoplasm Staging
RNA, Messenger/genetics/metabolism
Real-Time Polymerase Chain Reaction
Signal Transduction
Stomach/metabolism/pathology
Stomach Neoplasms/*metabolism/pathology
Up-Regulation
Adrenergic beta-Agonists
Cell Adhesion Molecules
Isoproterenol
RNA, Messenger
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