J Genet Med.  2016 Dec;13(2):89-94. 10.5734/JGM.2016.13.2.89.

The activity of factor VIII and IX of cord blood at mid-trimester in fetuses without hemophilia

• Choe DK
• Oh JW
• Jun JK
• Choi YM

• KMID: 2369025
• DOI: http://doi.org/10.5734/JGM.2016.13.2.89

Abstract

PURPOSE
Molecular genetic analysis is the main approach used for prenatal diagnosis of hemophilia A and B. However, in certain cases, such analysis is uninformative. In such situations, direct measurement of fetal coagulation factor levels is still the best option, and it may be the only option in some cases. This study was conducted to determine the normal ranges of midtrimester cord blood factor VIII (FVIII) and IX (FIX) in a Korean population.
MATERIALS AND METHODS
Twenty-six FVIII samples and 29 FIX samples were assayed in fetal cord blood acquired by ultrasound-guided cordocentesis. Sampling was conducted during gestational ages of 19-24 weeks.
RESULTS
The mean and standard deviations for FVIII and FIX activity were 45.5±30.5% and 19.9±12.2%, respectively. Ranges for FVIII and FIX were 1.5-125.0% and 6.0-52.0%, respectively.
CONCLUSION
Our study revealed the normal ranges and lowest level of factor VIII and factor IX in non-affected normal fetus by fetal cord blood sampling during the mid-trimester in a Korea population. The factor assay of the fetal cord blood is invasive but feasible and provides important basic data related to hemophilia.

Keyword

Hemophilia; Blood coagulation factors; Cordocentesis; Prenatal diagnosis

MeSH Terms

Blood Coagulation Factors
Cordocentesis
Factor IX
Factor VIII*
Female
Fetal Blood*
Fetus*
Gestational Age
Hemophilia A*
Humans
Korea
Molecular Biology
Pregnancy
Pregnancy Trimester, Second
Prenatal Diagnosis
Reference Values
Blood Coagulation Factors
Factor IX
Factor VIII