J Korean Med Sci.  2015 Dec;30(12):1784-1792. 10.3346/jkms.2015.30.12.1784.

Urinary Nucleic Acid TSPAN13-to-S100A9 Ratio as a Diagnostic Marker in Prostate Cancer

Affiliations
  • 1Department of Urology, College of Medicine, Chungbuk National University, Cheongju, Korea. wjkim@chungbuk.ac.kr
  • 2Bio-Medical Science Co. Ltd, Daejeon, Korea.
  • 3Nucleic Acid Research Center, Inc., Daejeon Oriental Hospital of Daejeon University, Daejeon, Korea.
  • 4Department of Biochemistry, Dong-Eui University, Busan, Korea.
  • 5Department of Food and Biotechnology, Chung-Ang University, Seoul, Korea.

Abstract

The potential use of urinary nucleic acids as diagnostic markers in prostate cancer (PCa) was evaluated. Ninety-five urine samples and 234 prostate tissue samples from patients with PCa and benign prostatic hyperplasia (BPH) were analyzed. Micro-array analysis was used to identify candidate genes, which were verified by the two-gene expression ratio and validated in tissue mRNA and urinary nucleic acid cohorts. Real-time quantitative polymerase chain reaction (qPCR) was used to measure urinary nucleic acid levels and tissue mRNA expression. The TSPAN13-to-S100A9 ratio was selected to determine the diagnostic value of urinary nucleic acids in PCa (P = 0.037) and shown to be significantly higher in PCa than in BPH in the mRNA and nucleic acid cohort analyses (P < 0.001 and P = 0.013, respectively). Receiver operating characteristic (ROC) analysis showed that the area under the ROC curve was 0.898 and 0.676 in tissue mRNA cohort and urinary nucleic acid cohort, respectively. The TSPAN13-to-S100A9 ratio showed a strong potential as a diagnostic marker for PCa. The present results suggest that the analysis of urine supernatant can be used as a simple diagnostic method for PCa that can be adapted to the clinical setting in the future.

Keyword

Prostatic Neoplasms; Cell-free Nucleic Acid; Urine; Two-gene Expression Ratio; S100A9; TSPAN13; Diagnostic Marker

MeSH Terms

Aged
Aged, 80 and over
Biomarkers, Tumor/*genetics/*urine
Calgranulin B/*genetics
Cohort Studies
Humans
Male
Middle Aged
Nucleic Acids/*genetics/*urine
Oligonucleotide Array Sequence Analysis
Prostate/metabolism
Prostatic Hyperplasia/diagnosis/genetics/urine
Prostatic Neoplasms/diagnosis/*genetics/*urine
RNA, Messenger/genetics/metabolism
RNA, Neoplasm/genetics/metabolism
ROC Curve
Real-Time Polymerase Chain Reaction
Tetraspanins/*genetics
Biomarkers, Tumor
Calgranulin B
Nucleic Acids
RNA, Messenger
RNA, Neoplasm
Tetraspanins

Figure

  • Fig. 1 Study design and validation strategies. GEO, Gene Expression Omnibus; PCa, prostate cancer; BPH, benign prostatic hyperplasia.

  • Fig. 2 Box plot of TSPAN13-to-S100A9 expression ratios in the tissue mRNA cohort and urinary nucleic acid cohort.

  • Fig. 3 ROC curve analysis of TSPAN13-to-S100A9 ratios in the tissue mRNA cohort and urinary nucleic acid cohort.


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