J Periodontal Implant Sci.  2016 Oct;46(5):350-359. 10.5051/jpis.2016.46.5.350.

Increased osteoinductivity and mineralization by minimal concentration of bone morphogenetic protein-2 loaded onto biphasic calcium phosphate in a rabbit sinus

• Kim JS
• Cha JK
• Lee JS
• Choi SH
• Cho KS

• KMID: 2354933
• DOI: http://doi.org/10.5051/jpis.2016.46.5.350

Abstract

PURPOSE
The purpose of the present study was to evaluate the effectiveness of a minimal concentration of bone morphogenetic protein-2 (BMP-2) in terms of quantitative and qualitative analyses of newly formed bone in a rabbit maxillary sinus model.
METHODS
In 7 rabbits, sinus windows were prepared bilaterally. Biphasic calcium phosphate (BCP) loaded with 0.05 mg/mL BMP-2 was grafted into one sinus (the BMP group) and saline-soaked BCP was placed into the other (the control group) in each animal. The animals were allowed an 8-week healing period before being sacrificed. Specimens including the augmented area and surrounding tissues were then removed and evaluated both radiographically and histologically.
RESULTS
There was a difference in the mineralization of new bone between the groups. In the BMP group, the greater part of the new bone consisted of mature lamellar bone with an evident trabecular pattern, whereas the control group showed mostly woven bone, consisting only partially of lamellar bone. Histometrically, the area of new bone was significantly greater (4.55±1.35 mm2 vs. 2.99±0.86 mm2) in the BMP group than in the control group (P<0.05); however, the total augmentation volumes were not significantly different between the groups.
CONCLUSIONS
Within the limitations of this study, it can be suggested that a minimal concentration of BMP-2 (0.05 mg/mL) had an osteoinductive effect with accelerated mineralization in a rabbit sinus model using a BCP carrier.

Keyword

Bone morphogenetic proteins; Physiologic calcification; Hydroxyapatite-beta tricalcium phosphate; Sinus floor augmentation

MeSH Terms

Animals
Bone Morphogenetic Proteins
Calcification, Physiologic
Calcium*
Maxillary Sinus
Miners*
Rabbits
Sinus Floor Augmentation
Transplants
Bone Morphogenetic Proteins
Calcium