J Cancer Prev.  2016 Jun;21(2):121-125. 10.15430/JCP.2016.21.2.121.

Human Papillomavirus Genotyping and p16INK4a Expression in Cervical Lesions: A Combined Test to Avoid Cervical Cancer Progression

• Zouheir Y
• Fechtali T
• Elgnaoui N

• KMID: 2353813
• DOI: http://doi.org/10.15430/JCP.2016.21.2.121

Abstract

Cervical cancer is a major public health problem in Morocco. The cervical cancer has a long precancerous period that provides an opportunity for the screening and treatment. Improving screening tests is a priority goal for the early diagnosis of cervical cancer. This study was conducted to evaluate the combination of p16INK4a protein expression, human papillomavirus (HPV) typing, and histopathology for the identification of cervical lesions with high risk to progress to cervical cancer among Moroccan women. A total of 96 cervical biopsies were included in this study. Signal amplification in situ hybridization with biotinylated probes was used to detect HPV. Immunohistochemistry was used to evaluate the expression of p16INK4a protein. HPV DNA was detected in 74.0% of the biopsies (71/96). Of the seventy-one positive HPV cases, we detected 67.6% (48/71) of high risk (HR)-HPV (HPV 16 and 18), 24% of low risk-HPV (HPV 6 and 11), 1.4% intermediate risk-HPV (HPV 31, 33, and 35), and 7% coinfections (HPV 6/11 and 16/18). Overexpression of p16INK4a protein was observed in 72.9% (70/96) of the biopsies. In addition, p16INK4a protein detection was closely correlated with recovery of HR HPV. Our result showed that p16INK4a expression level is correlated with HR-HPV status.

Keyword

Human papillomavirus; Biopsy; Cyclin-dependent kinase inhibitor p16; Immunohistochemistry; In situ hybridization

MeSH Terms

Biopsy
Coinfection
Cyclin-Dependent Kinase Inhibitor p16
DNA
Early Diagnosis
Female
Humans*
Immunohistochemistry
In Situ Hybridization
Mass Screening
Morocco
Public Health
Uterine Cervical Neoplasms*
Cyclin-Dependent Kinase Inhibitor p16
DNA