J Korean Geriatr Soc.  2008 Dec;12(4):235-245.

Expression Change of Complement Regulator Genes of the Human Astrocytoma Cell Line after A beta1-42 and Interferon Gamma Administration

• Choi YS
• Kim SH

Abstract

BACKGROUND: We determined the changes of complement regulator gene expression in the amyloid-beta1-42(A beta1-42) and interferon-gamma(IFN-gamma)-stimulated human astrocytoma cell line.
METHODS
The human astrocytoma cell line, U373MG, was stimulated with IFN-gamma(62.5-1,000U/ml) in the presence or absence of aggregated A beta1-42(1-20micrometer) for 24 hours. Messenger RNA expression of C1 inhibitor(C1-INH), complement factor I(CFI), clusterin, vitronectin, decay accelerating factor(DAF), membrane cofactor protein(MCP), and CD59 was measured by quantitative real-time reverse transcriptase-PCR.
RESULTS
IFN-gamma(final concentration, 500U/ml) markedly increased the expression of mRNA for C1-INH in a time dependent fashion. A beta1-42(final concentration, 2micrometer) induced a slight increase in the expression of C1-INH. Messenger RNAs for CFI and clusterin were minimally increased, but other regulators were unchanged or decreased by either A beta1-42 or IFN-gamma. IFN-gamma overrode A beta1-42-induced mRNA expression of C1-INH when the cells were treated with these two reagents together.
CONCLUSION
Among the complement regulator genes in the human astrocytoma cell line, U373MG, only C1-INH was significantly up-regulated by IFN-gamma with or without A beta1-42 administration.

Keyword

Alzheimer's Disease; beta-amyloid protein; C1 Inhibitor; Complement Factor I; Interferon-gamma

MeSH Terms

Alzheimer Disease
Aminopeptidases
Amyloid beta-Peptides
Astrocytoma
Cell Line
Clusterin
Complement Factor I
Complement System Proteins
Genes, Regulator
Humans
Indicators and Reagents
Interferon-gamma
Interferons
Membranes
RNA, Messenger
Vitronectin
Aminopeptidases
Amyloid beta-Peptides
Clusterin
Complement Factor I
Complement System Proteins
Indicators and Reagents
Interferon-gamma
Interferons
RNA, Messenger
Vitronectin