J Korean Med Sci.
1992 Sep;7(3):189-192. 10.3346/jkms.1992.7.3.189.
Transcription of class II MHC gene by interferon-gamma in FRTL-5 cells
- Affiliations
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- 1Department of Internal Medicine, Korea Cancer Center Hospital, Seoul, Korea.
- KMID: 1421913
- DOI: http://doi.org/10.3346/jkms.1992.7.3.189
Abstract
- The intracellular mechanism by which interferon-gamma induces the expression of class II major histocompatibility complex (MHC) antigen in nonlymphoid cells is not clear. The effect of recombinant rat interferon-gamma (IFN-gamma), and cycloheximide on the expression of class II MHC gene was studied using the techniques of immunocytochemical staining and northern blot analysis. IFN-gamma induced de novo transcription of class II MHC gene and class II MHC antigen expression on the cell surface. Cycloheximide did not inhibit IFN-gamma-induced class II MHC antigen expression in a dose-dependent manner indicating translational blockade. These results suggest that IFN-gamma induces class II MHC antigen expression via de novo transcription of class II MHC gene leading to synthesis of new class II MHC molecule.
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