Exp Mol Med.
1998 Jun;30(2):93-99.
Partial purification and characterization of a novel murine factor that augments
the expression of class I MHC antigens on tumor cells
- Affiliations
-
- 1School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Abstract
- A soluble factor which augments the expression of major histocompatibility
complex class I (MHC I) antigens on a number of murine tumor cell lines, has
been isolated from the culture supernatants of mixed lymphocyte reaction of
spleen cells derived from C57B1/6, Balb/c and Swiss mice. The factor, termed
MHC-augmenting factor (MHC-AF) has been partially purified by Sephadex G-100
column chromatography and reverse phase HPLC. MHC-AF activity is associated with
an 18 kDa molecule. MHC-AF activity was resistant to pH 2.0 treatment and
partially purified MHC-AF preparations did not have any activity in L929
cell/vesicular stomatitis virus (VSV) interferon bioassay system. Antibodies to
IFN-gamma did not block the activity of MHC-AF. These results indicate that a
MHC-AF distinct from IFN-gamma, is produced by mouse spleen cells undergoing a
mixed lymphocyte reaction.