Korean J Ophthalmol.  1994 Dec;8(2):66-71. 10.3341/kjo.1994.8.2.66.

Expression of major histocompatibility complex antigen in Lewis rat cornea

• Tchah H

• KMID: 1735126
• DOI: http://doi.org/10.3341/kjo.1994.8.2.66

Abstract

Fresh rat corneas as well as corneas preserved in several different corneal preservation media were stained with Avidin-Biotin-peroxidase Complex method in order to evaluate major histocompatibility complex (MHC) antigen expression. In fresh corneas, class I antigen was identified in corneal epithelium, stroma and endothelium. Class II antigen was identified only in stroma. In corneas preserved in the media which contained chondroitin and dextran for 7 days, class I antigen was somewhat decreased but class II antigen was increased. In corneas preserved in the medium which contained insulin or epidermal growth factor for 7 days, class II antigens seemed to be increased compaired to the fresh cornea. Expression of MHC antigens of corneas in the medium with fetal bovine serum were similar to those of fresh corneas.

Keyword

cornea; major histocompatability complex antigen; expression; corneal preservation media

MeSH Terms

Animals
Cornea/*metabolism
Culture Media
Histocompatibility Antigens Class I/*biosynthesis
Histocompatibility Antigens Class II/*biosynthesis
Immunoenzyme Techniques
Major Histocompatibility Complex
Organ Preservation/methods
Rats
Rats, Inbred Lew
Culture Media
Histocompatibility Antigens Class I
Histocompatibility Antigens Class II