Korean J Ophthalmol.  1997 Jun;11(1):32-38. 10.3341/kjo.1997.11.1.32.

The effect of growth factor on antigen and viability of corneal endothelial cells

• Tchah H

• KMID: 754195
• DOI: http://doi.org/10.3341/kjo.1997.11.1.32

Abstract

The effect of epidermal growth factor (EGF) on corneal endothelial cell of rabbit, was evaluated. DNA synthesis of cultured corneal endothelial cells after adding EGF to culture medium and major histocompatibility (MHC) antigen expression of cornea preserved in EGF added Optisol were measured. EGF increased DNA synthesis of corneal endothelial cells at all concentrations tested (0.03 ng/ml-300 ng/ml) compared with control. The maximum increase of DNA synthesis was shown at the concentrations of 3.0-30 ng/ml. The effect of EGF on increase of DNA synthesis was more profound in second sub-cultured cells compared with first sub-cultured cells. In corneas preserved in Optisol for 3 days, 4 degrees C, class I antigen expression is decreased while class II antigen expression was slightly increased. In corneas preserved in EGF added Optisol for 3 days, 4 degrees C, class II antigen expression was more increased compared with those without EGF.

MeSH Terms

Animal
Cell Survival/drug effects*
Cells, Cultured
Culture Media, Serum-Free/pharmacology
DNA/drug effects
DNA/biosynthesis*
DNA Replication/drug effects
Endothelium, Corneal/drug effects*
Endothelium, Corneal/cytology
Epidermal Growth Factor/pharmacology*
Histocompatibility Antigens Class I/metabolism*
Histocompatibility Antigens Class II/metabolism*
Rabbits
Rats
Rats, Inbred Lew
Substances: Optisol
Substances: DNA
Substances: Epidermal Growth Factor
Substances: Histocompatibility Antigens Class II
Substances: Histocompatibility Antigens Class I
Substances: Culture Media, Serum-Free