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Purification and characterization of recombinant murine endostatin in E. coli

You WK, So SH, Lee H, Park SY, Yoon MR, Chang SI, Kim HK, Joe YA, Hong YK, Chung SI

  • KMID: 1029927
  • Exp Mol Med.
  • 1999 Dec;31(4):197-202.
Endostatin, a carboxyl-terminal fragment of collagen XVIII is known as an anti-angiogenic agent, that specifically inhibits the proliferation of endothelial cell and the growth of several primary tumor....
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Partial characterization of a 17 kDa protein of Clonorchis sinensis

Chung YB, Chung BS, Choi MH, Chai JY, Hong ST

A 17 kDa protein from Clonorchis sinensis adults was purified by a procedure including Sephacryl S-200 HR gel filtration and Q-Sepharose anion exchange chromatography. The protein was proved to be...
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Reconstitution of class I MHC molecules expressed in E. coli and complexed with single antigenic peptides

Kim J, Kim MY, Chwae YJ, Park JH

The HLA-Cw3 heavy chain has been expressed at high level as insoluble protein aggregates in E. coli. The protein aggregates dissolved in strong denaturant solution were efficiently reconstituted by...
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Identification and purification of IgE-reactive proteins in German cockroach extract

Yong TS, Lee JS, Lee J, Park SJ, Jeon SH, Ree HI, Park JW

Cockroaches have been implicated as a cause of respiratory allergy in urban areas worldwide. IgE-reactive German cockroach proteins were identified with molecular weights (MWs) of 90, 66, 50,...
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Topical fibronectin treatment in persistent corneal epithelial defects and corneal ulcers

Kim KS, Oh JS, Kim IS, Jo JS

Topical fibronectin, autologous and homologous, was used to treat nine patients (eleven eyes) with persistent corneal epithelial defects and corneal ulcers that failed to improve with standard therapy. The fibronectin...
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A new member of alpha 1-adrenoceptor-coupled G alpha h (transglutaminase II) family in pig heart: purification and characterization

Yoo SM, Jeong HS, Han KJ, Cho SH, Lee HS, Yun HY, Kwon NS, Baek KJ

  • KMID: 754992
  • Exp Mol Med.
  • 1998 Jun;30(2):81-86.
We previously reported an identification of a 77-kDa GTP-binding protein that co-purified with the alpha 1-adrenoceptor following ternary complex formation. In the present paper, we report on the purification and characterization of...
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Charting the proteome of Cryptosporidium parvum sporozoites using sequence similarity-based BLAST searching

Siddiki AM, Wastling J

Cryptosporidium (C.) spp. are important zoonotic parasites causing widespread diarrhoeal disease in man and animals. The recent release of the complete genome sequences for C. parvum and C. hominis has...
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Partial purification and characterization of a novel murine factor that augments the expression of class I MHC antigens on tumor cells

Puri N, Saxena RK

  • KMID: 754994
  • Exp Mol Med.
  • 1998 Jun;30(2):93-99.
A soluble factor which augments the expression of major histocompatibility complex class I (MHC I) antigens on a number of murine tumor cell lines, has been isolated from the culture supernatants of...
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Expression of a recombinant branched chain alpha-oxo acid dehydrogenase complex E2 (BCOADC-E2) in insect cells and its immunoreactivity to autoimmune sera

Lee SM, Ko SH, Park JE, Cha SH

  • KMID: 754990
  • Exp Mol Med.
  • 1998 Jun;30(2):65-71.
Preparation of a pure autoantigen by way of recombinant DNA technology has an important value in an accurate diagnosis or prognosis of an autoimmune disease. BCOADC-E2 subunit, a mitochondrial protein, has been...
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Functional activities of the Tsh protein from avian pathogenic Escherichia coli (APEC) strains

Kobayashi , Gaziri LC, Vidotto MC

The temperature-sensitive hemagglutinin (Tsh) expressed by strains of avian pathogenic Escherichia (E.) coli (APEC) has both agglutinin and protease activities. Tsh is synthesized as a 140 kDa precursor protein, whose...
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A Study of the Regulation of the Glucose Transporter in the Plasma Membranes of Hepatoma Cells Induced by 3'-Me DAB

Ahn YH, Chai KJ, Kim SN, Kim YS

5'-nucelotidase and glucose-6-phosphatase are liver plasma and microsomal membranes markers and their respective activities were determined. In the liver homogenate, the activities of 5'-nucleotidase were 0.58 +/- 0.08 and 0.29...
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Biological and biochemical modulation of Trichomonas vaginalis KT9 isolate after shifting of culture medium from TPS-1 into TYM

Ryu JS, Choi R, Park SY, Park H, Min DY

To evaluate the biological and biochemical characteristics of Trichomonas vaginalis KT9 isolate, the growth and size of trichomonads, pathogenicity in mouse, protein profiles and proteinase activity were examined after...
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