Yonsei Med J.  1999 Jun;40(3):283-289. 10.3349/ymj.1999.40.3.283.

Identification and purification of IgE-reactive proteins in German cockroach extract

• Yong TS
• Lee JS
• Lee J
• Park SJ
• Jeon SH
• Ree HI
• Park JW

• KMID: 711649
• DOI: http://doi.org/10.3349/ymj.1999.40.3.283

Abstract

Cockroaches have been implicated as a cause of respiratory allergy in urban areas worldwide. IgE-reactive German cockroach proteins were identified with molecular weights (MWs) of 90, 66, 50, 43 and 36 KD by immunoblot analysis in both immune BALB/c mice and sensitized humans. Prominent IgE-reactive proteins were purified using FPLC by ion-exchange chromatography, gel filtration and hydrophobic chromatography. The N-terminal amino acid sequence of a purified protein with a MW of 66 KD on SDS-PAGE was Val-Thr-Leu-Lys-Lys(Val)-Met-Ile-Lys-Thr-Phe-Tyr. No homologous protein was found through a search of GenBank that indicated a novel IgE-reactive protein in German cockroach extract. Another purified protein with a MW of 36 KD reacted strongly with a monoclonal antibody against Bla g 2.

Keyword

German cockroach; IgE; allergens; immunoblot; amino acid sequence

MeSH Terms

Amino Acid Sequence/genetics
Animal
Cockroaches/chemistry*
Human
IgE/immunology*
Insect Proteins/isolation & purification*
Insect Proteins/immunology*
Insect Proteins/genetics
Mice
Mice, Inbred BALB C
Tissue Extracts/chemistry*