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Recommendations for the Use of Liquid Chromatography-Mass Spectrometry in the Clinical Laboratory: Part I. Implementation and Management

Lee K, Moon SY, Kim S, Choi HJ, Lee SG, Park HD, Lee SY, Song SH, Clinical Mass Spectrometry Research Committee of the Korean Society of Clinical Chemistry

Many types of assays involving mass spectrometry have been developed and applied in clinics. However, mass spectrometry has not been widely implemented yet relative to other measurement methods, including biochemical...
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Quality of Same-Day Sputum Smears Microscopy and Presumptive Tuberculosis Patients Drop-out at Health Facilities of Addis Ababa, Ethiopia

Taddese BD, Misganaw A

BACKGROUND: Same-day sputum microcopy is recommended in areas where sputum smear microscopy external quality assessment (EQA) is effectively implemented and sturdy. In Addis Ababa, the status of EQA and drop-out...
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Design and Laboratory Implementation of Web Application for Collaboratively Setting Reference Intervals

Jeon BR, Lee YW

BACKGROUND: Although the same equipment and reagents can be employed for inspecting identical samples, the setting and verification methods for the corresponding reference intervals differ from each other, and such...
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Traditional and Novel Mechanisms of Heat Shock Protein 90 (HSP90) Inhibition in Cancer Chemotherapy Including HSP90 Cleavage

Park S, Park JA, Jeon JH, Lee Y

HSP90 is a molecular chaperone that increases the stability of client proteins. Cancer cells show higher HSP90 expression than normal cells because many client proteins play an important role in...
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Use of Liquid Chromatography-Tandem Mass Spectrometry for Clinical Testing in Korean Laboratories: a Questionnaire Survey

Chae H, Cho SE, Park HD, Chun S, Lee YW, Yun YM, Song SH, Lee SG, Lee K, Song J, Lee SY, On behalf of the Clinical Mass Spectrometry Research Committee of Korean Society of Clinical Chemistry

BACKGROUND: The use of liquid chromatography-tandem mass spectrometry (LC-MS/MS) has substantially increased in clinical laboratories worldwide. To assess the status of clinical LC-MS/MS testing in Korean laboratories, a questionnaire survey...
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Systemic and Local Phenotypes of Barium Chloride Induced Skeletal Muscle Injury in Mice

Jung HW, Choi JH, Jo T, Shin H, Suh JM

Skeletal muscle regeneration in mice has traditionally been studied using local freeze burn or snake venom injection models. More recently, a barium chloride (BaClâ‚‚)-induced muscle injury model has been established...
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Analyses of the Proficiency Testing Program and the Performance of Qualitative Reagents for Rheumatoid Factor

Oh J, Park HD

BACKGROUND: Rheumatoid factor (RF) is used as one of the diagnostic criteria for rheumatoid arthritis. The purpose of this study was to evaluate qualitative RF reagents used in clinical laboratories...
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Annual Report of the Korean Association of External Quality Assessment Service on Urinalysis and Fecal Occult Blood Testing (2018)

Jeon CH, Lee AJ, Kim SG, Suh HS, Bae YC

In 2018, external quality assessment trials for urinalysis and fecal occult blood (FOB) were performed using 1,590 participants. Urine chemistry tests were performed thrice while urine sediment and FOB tests...
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Performance Evaluation of SENTiFIT 270 and FOB Gold Reagent for Detecting Fecal Occult Blood

Kang DY, Kim D, Kim K, Jang IH, Jeong SH

BACKGROUND: Fecal occult blood tests have been widely used to screen for colorectal cancer. SENTiFIT 270 (Sentinel diagnostics, Italy) is a fecal occult blood test with an immunochemical method that...
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Performance Evaluation of the Stat Profile pHOx Ultra Blood Gas Analyzer

Lee HY, Ahn S, Kim HK, Lee W, Chun S, Min WK

The objective of this study was to evaluate the analytical performance of the Stat Profile pHOx Ultra Blood Gas Analyzer (Nova Biomedical, USA), a new blood gas/chemistry analyser, including its...
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Accuracy-Based Proficiency Testing of Creatinine Measurement: 7 Years' Experience in Korea

Jeong TD, Lee HA, Lee K, Yun YM

BACKGROUND: Standardization of creatinine assay is consistently performed and much effort has been put into improving the accuracy of the results. We aimed to analyze the results of accuracy-based proficiency...
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Capsaicin-Induced Apoptosis in MBT-2 Murine Bladder Tumor Cells and Bladder Wall Penetration Effect of the Nano-Encapsulated Capsaicin in Rabbit

Cho SJ, Moon HW, Bae WJ, Park YH, Ha US, Hong SH, Kim SW, Lee JY

PURPOSE: In this study, we attempted to characterize capsaicin's effects with regard to the apoptosis of murine bladder cancer cells (MBT-2) as well as the pharmacodynamics of nano-encapsulated capsaicin formulation...
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The First Case of Para-Bombay Blood Type Encountered in a Korean Tertiary Hospital

Kim MS, Kim JS, Park H, Chung Y, Kim H, Ko DH, Kim SH, Hwang SH, Oh HB

Para-Bombay phenotypes are rare blood groups that have inherent defects in producing H antigens associated with FUT1 and/or FUT2. We report the first case of para-Bombay blood type in a...
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Performance Evaluation of the CRE2 Reagent from Siemens for Serum Creatinine Measurement

Kim H, Lim J, Koo SH, Kim J, Kim SY, Kwon GC

BACKGROUND: For creatinine measurement, the enzymatic method is known to be more accurate than the Jaffe method; however, the latter is still widely used. We evaluated the performance of the...
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Factors Affecting Hemagglutinations Strength in ABO Blood Group Typing Test Using the Tube Method

Jeon YL, Lee WI, Kang SY, Kim MH

BACKGROUND: The ABO blood group typing test (ABO test) is an initial pre-transfusion test based on hemagglutination. Although various factors affect hemagglutination strength, few studies have examined how these factors...
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Annual Report on the External Quality Assessment Scheme for Urinalysis and Fecal Occult Blood Testing in Korea (2017)

Jeon CH, Lee AJ, Kim SG, Suh HS, Bae YC

In 2017, external quality assessment trials for urinalysis and fecal occult blood (FOB) were performed with 1,544 participants. Urine chemistry tests were performed three times and urine sediment and FOB...
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Postprandial Lipid Concentrations and Daytime Biological Variation of Lipids in a Healthy Chinese Population

Yang D, Cai Q, Qi X, Zhou Y

BACKGROUND: Several latest guidelines and consensus statements from Europe and the United States specify that there is no need for fasting prior to routine lipid tests. However, the latest Chinese...
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IgE Cross-Reactivity between Humulus japonicus and Humulus lupulus

Jeong KY, Lee J, Mistrello , Park KH, Park JW

PURPOSE: Japanese hop (Humulus japonicus) is a major cause of weed pollinosis in East Asia. However, supplies of commercial allergen extract from this plant have not met clinical demand. The...
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Allergen standardization

Park JW, Jeong KY

Allergen immunotherapy (AIT) and diagnostic tests are based on well qualified allergen extracts, which are derived from biologic organisms. The allergenicity of the extracts is markedly affected by the climate,...
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Inhibition of the 26S and 20S Proteasomes by the Site-specific Inhibitors

Woo KM

OBJECTIVE: The study was performed to establish the purification processes of both 26S and 20S proteasomes, also to investigate the inhibitory properties and patterns of two different proteasome inhibitors on...
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