J Korean Geriatr Soc.  2006 Dec;10(4):278-284.

Subcellular Distribution of Microtubule in Artificially Induced Senescent PC12 Cells

• Lee E
• Oh CS
• Shin DH
• Lee YS

Abstract

Background: Since recent reports showed the possibility that cytoskeletal proteins, which were known to be exclusively within the cytoplasm, might play a role in the re-distribution of the intranuclear chromatin in a certain type of the cells under specific circumstances, we tried to show a change in the intracellular distribution of microtubule protein in artificially induced senescent PC12 pheochromocytoma cells.
Methods
PC12 pheochromocytoma cells (ATCC CRL-1721) were grown in the culture media including 1 micrometer 3'-Azido-3'-deoxythymidine (AZT, Sigma-Aldrich, USA). The senescence of the cells was confirmed by senescence detection kit (Calbiochem, San Diego, CA). Immunocytochemical study was also performed in the cells treated with AZT during 0, 75 and 153 days.
Results
beta-tubuline was not observed in the cells not treated with AZT. The same protein was localized within the nuclei in the senescent cells treated with AZT during 153 days.
Conclusion
Microtubule might be involved in some crucial roles in the redistribution of chromatin within the nuclei of the senescent cells.

Keyword

Microtubule; PC12; Cellular senescence; AZT; Tubuline

MeSH Terms

Aging
Animals
Cell Aging
Chromatin
Culture Media
Cytoplasm
Cytoskeletal Proteins
Microtubules*
PC12 Cells*
Pheochromocytoma
Tubulin
Zidovudine
Chromatin
Culture Media
Cytoskeletal Proteins
Tubulin
Zidovudine