J Korean Geriatr Soc.  2006 Dec;10(4):285-289.

Distribution Change of WD40 Repeat Protein 1 in Artificially Induced Senescent PC12 Cells

• Shin DH
• Oh CS
• Lee E
• Oh SH
• Lee YS

Abstract

Background: WDR1 is thought to be correlating with polymerization and depolymerization of actin protein. Though WDR1 was found to be within nucleus, in which actin could not be present by previous studies, the exact distribution pattern of WDR1 protein under various circumstances was not elucidated up to the present time. In this regard, we tried to see a change in the distribution of WDR1 protein within artificially induced senescent PC 12 pheochromocytoma cells for the first time.
Methods
PC12 pheochromocytoma cells (ATCC CRL-1721) were grown in the culture media including 1 micrometer 3'-Azido-3'-deoxythymidine (AZT, Sigma-Aldrich, USA). The senescence of the cells was confirmed by senescence detection kit (Calbiochem, San Diego, CA). Immunocytochemical study by using WDR1 antibody was also performed in the cells treated with AZT during 0, 75 and 153 days.
Results
WDR1 protein was mainly observed within the cytoplasm of the cells not treated with AZT. However, the distribution of the same protein was changed into the nucleus after 153 day-AZT treatment.
Conclusion
The distribution of WDR1 protein was changed into nucleus in the artificially senescent PC12 cells.

Keyword

WD40 repeat protein 1; PC12; Senescence; 3'-Azido-3'-deoxythymidine

MeSH Terms

Actins
Aging
Animals
Culture Media
Cytoplasm
PC12 Cells*
Pheochromocytoma
Polymerization
Polymers
Zidovudine
Actins
Culture Media
Polymers
Zidovudine