Korean J Parasitol.
2007 Sep;45(3):229-232. 10.3347/kjp.2007.45.3.229.
Metacercarial proteins interacting with WD40-repeat protein of Clonorchis sinensis
- Affiliations
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- 1Department of Parasitology, Chung-Ang University College of Medicine, Seoul, Korea. hongsj@cau.ac.kr
- 2Division of Malaria and Parasitic Diseases, National Institute of Health, Korea Centers for Disease Control and Prevention, Seoul, Korea.
- 3Department of Parasitology, Yanbin University College of Medicine, Yanji, Jilin, P.R. China.
- 4Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul, Korea.
- 5Department of Biochemistry, School of Natural Sciences, Chungbuk National University, Cheongju, Korea.
- KMID: 1104535
- DOI: http://doi.org/10.3347/kjp.2007.45.3.229
Abstract
- The WD40-repeat proteins serve as a platform coordinating partner proteins and are involved in a range of regulatory cellular functions. A WD40-repeat protein (CsWD1) of Clonorchis sinensis previously cloned is expressed stage-specifically in the tegumental syncytium of C. sinensis metacercariae. In the present study, interacting proteins with the CsWD1 protein was purified by immunoprecipitation and 2 dimension gel electrophoresis from the C. sinensis metacercaria soluble extract, and tryptic peptides were analyzed by LC/ESI-MS. Putative partner proteins were annotated to be actin-2, glyceraldehyde-3-phosphate dehydrogenase, and hypothetical and unmanned proteins. The CsWD1 protein was predicted to contain 3 conserved actin-interacting residues on its functional surface. With these results, the CsWD1 protein is suggested to be an actin-interacting protein of C. sinensis.
Keyword
MeSH Terms
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Animals
Antibodies, Helminth/metabolism
Clonorchis sinensis/*physiology
Electrophoresis, Gel, Two-Dimensional/veterinary
Helminth Proteins/chemistry/*isolation & purification/metabolism
Hydrogen-Ion Concentration
Immunoglobulin G/chemistry
Microfilament Proteins/chemistry/*isolation & purification/metabolism
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