J Korean Pediatr Soc.
2002 Feb;45(2):247-255.
Megakaryocyte Colony Formation of Fetal Liver Cells
- Affiliations
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- 1Department of Pediatrics, Sungkyunkwan University, College of Medicine, Masan, Korea. chunsoo7@unitel.co.kr
- 2Department of Clinical Pathology, Keimyung University, College of Medicine, Taegu, Korea.
- 3Department of Obstetrics & Gynecology, Keimyung University, College of Medicine, Taegu, Korea.
- 4Department of Pediatrics, Keimyung University, College of Medicine, Taegu, Korea.
Abstract
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PURPOSE: This study was undertaken to obtain basic data about the megakaryocyte colony formation of fetal liver cells by using immunocytochemical staining and ex vivo culture with growth factors.
METHODS
The mononuclear cells were isolated from fetal liver and bone marrow with idiopathic thrombocytopenic purpura(ITP) and pancytopenia. These mononuclear cells were cultured in MegaCultTM-C(Stem Cell Tech, Canada) media in the presence of growth factors and CFU-Megakaryocyte(CFU-Mk) colonies were counted on day 12. The expansion of CD34+ and CD41+ cell was analyzed by flow cytometry after 5 days incubation using flask culture.
RESULTS
The numbers of CFU-Mk colonies of mononuclear cells obtained from fetal liver in the 11th week gestational age were more than those in the 19th week specimens; growth factors could not enhance the colony expansion in all cases. Total numbers of CFU-Mk colony of fetal liver cells were higher than bone marrow from ITP or pancytopenia groups. The numbers of pure or large CFU-Mk colonies of fetal liver cells were also higher than bone marrow specimens. The rate of CD34+ cell expression of fetal liver was increased after flask culture and the enhancement effect of epression was seen only in cases which added thrombopoietin. The rate of CD41+ cell expression of fetal liver was increased after incubation, but the enhancement effect of growth factors was unclear.
CONCLUSION
This study revealed good results about the megakaryocyte colony assay of fetal liver mononuclear cells using MegaCultTM-C media. This study suggests that the fetal liver could be a good source of megakaryocytic progenitor cells for clinical application in hematopoietic stem cell transplantation.
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