Korean J Hematol.
1999 Aug;34(3):389-395.
Hematopoietic Stem Cell Colony Formation of the Fetal Liver
- Affiliations
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- 1Department of Pediatrics, Keimyung University, School of Medicine, Taegu, Korea.
- 2Institute for Medical Science, Keimyung University, School of Medicine, Taegu, Korea.
- 3Institute of Medical Genetics, Keimyung University, School of Medicine, Taegu, Korea.
- 4Department of Pediatrics, School of Medicine, Sungkyunkwan University, Samsung Hospital Masan, Korea.
- 5Kim's Clinic of Obstetrics and Gynecology, Taegu, Korea.
- 6Lee's Clinic of Obstetrics and Gynecology, Taegu, Korea.
Abstract
- BACKGROUND
Hematopoietic stem cells of the human fetal liver prior to 15 weeks gestation have remakable advantages for successful engraftment due to embryological immune immaturity, especially in-utero transplantation. This study was undertaken to obtain objective assessment data about the possibility of fetal liver hematopoietic stem cell transplantation in the future.
METHODS
Six cases of the fetal liver tissue were obtained from therapeutic abortions at 12~20 weeks gestation. The fetal liver was collected in RPMI media containing 10% fetal calf serum and the cell suspensions were obtained by centrifugation following physical disruption. The number of nucleated cells in each case was counted and the colony numbers in methyl cellulose media were scored according to incubation period with or without growth factors. Some of the cells were cryopreserved in the liquid nitrogen tank, thereafter cell viability and colony numbers were evaluated according to cryopreservation period.
RESULTS
The nucleated cell numbers obtained from each fetal liver increased with gestational age. The colony numbers after incubation increased with gestational age and the erythroid lineage was predominant in 3 cases which are under 15 weeks gestation. The colonogenic activity after incubation with combination of hematopoietic growth factors increased in only one case. The cell viability and the colony numbers after cryopreservation was decreased compare to the value before cryopreservation.
CONCLUSION
The number of nucleated cells and hematopoietic stem cell colony formation were increased with gestational age and viability of the cells after cryopreservation was decreased. Further systematic studies using more cases would be needed to obtain objective assessment data for fetal liver transplantation program in the future.
Keyword
MeSH Terms
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Abortion, Therapeutic
Cell Count
Cell Survival
Centrifugation
Cryopreservation
Female
Gestational Age
Hematopoietic Stem Cell Transplantation
Hematopoietic Stem Cells*
Humans
Intercellular Signaling Peptides and Proteins
Liver Transplantation
Liver*
Methylcellulose
Nitrogen
Pregnancy
Suspensions
Intercellular Signaling Peptides and Proteins
Methylcellulose
Nitrogen
Suspensions
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