Korean J Phys Anthropol.  2016 Jun;29(2):71-79. 10.11637/kjpa.2016.29.2.71.

Kainic Acid Treatment Increases Ca²⁺-mediated Neurotoxicity in the Mouse Hippocampus

• Lee JE

• KMID: 2328103
• DOI: http://doi.org/10.11637/kjpa.2016.29.2.71

Abstract

Kainic acid (KA)-induced neuronal cell death is associated with intracellular Ca²âº influx. However, it is unknown whether Lyn/Btk pathway is involved in the Ca²âº-mediated neurotoxicity and neuronal death induced by KA. In the present study, we investigated the altered expression of Ca²âº-controlled proteins in KA-treated hippocampus. Mice were sacrificed at 24 h after KA (20 mg/kg) systemic injection. We conducted Electroencephalographic (EEG) recording and examined hippocampal alterations by Western blotting and immunostaining in control mice or KA-treated mice. EEG tests showed that KA-treated mice increased seizure frequency and severity compared with control mice during KA-induced seizures. We found that KA decreases hippocalcin and calpain-mediated proteolysis in the hippocampus. In particular, the phosphorylation of Lyn and Btk was increased in KA-treated hippocampus compared to those of control mice. Our findings identify tyrosine kinases such as Lyn/Btk as a critical regulator of Ca²âº-mediated neurotoxicity in KA-induced seizures.

Keyword

Kainic acid; Calcium; Lyn/Btk; Seizure; Hippocampus

MeSH Terms

Animals
Blotting, Western
Calcium
Cell Death
Electroencephalography
Hippocalcin
Hippocampus*
Kainic Acid*
Mice*
Neurons
Phosphorylation
Phosphotransferases
Proteolysis
Seizures
Tyrosine
Calcium
Hippocalcin
Kainic Acid
Phosphotransferases
Tyrosine